Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):190-193. doi: 10.1099/ijs.0.020610-0. Epub 2010 Feb 26.
A novel actinobacterial strain, designated CPCC 201356(T), was isolated from a rhizosphere soil sample of the medicinal plant Astragalus membranaceus and subjected to a polyphasic taxonomic analysis. Good growth occurred at 20-32 °C, at pH 7.0-7.5 and with 0-1 % (w/v) NaCl. Colonies on R2A and ISP 2 agar were light red to red, round and lacked aerial mycelium; cells adhered to the agar. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(4)) and MK-9. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The major cellular fatty acids were iso-C(16 : 0), iso-C(15 : 0) and C(17 : 1)ω8c. The G+C content of the genomic DNA was 72.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CPCC 201356(T) belonged to the family Geodermatophilaceae and consistently formed a distinct sub-branch with Geodermatophilus obscurus DSM 43160(T). The organism showed 16S rRNA gene sequence similarity of 97.7 % with G. obscurus DSM 43160(T). DNA-DNA hybridization between strain CPCC 201356(T) and G. obscurus DSM 43160(T) was 17.4 %. On the basis of evidence from this polyphasic taxonomic study, a novel species, Geodermatophilus ruber sp. nov., is proposed; the type strain is CPCC 201356(T) (=DSM 45317(T) =CCM 7619(T)).
一株新的放线菌菌株，命名为 CPCC 201356(T)，从药用植物黄芪的根际土壤样本中分离得到，并进行了多相分类学分析。该菌在 20-32°C、pH7.0-7.5 和 0-1%（w/v）NaCl 的条件下生长良好。在 R2A 和 ISP 2 琼脂上的菌落呈浅红色到红色，圆形，缺乏气生菌丝；细胞黏附在琼脂上。肽聚糖含有meso-二氨基庚二酸作为诊断二氨基酸。主要的类异戊二烯醌为 MK-9(H(4)) 和 MK-9。极性脂类由双磷脂酰甘油、磷脂酰乙醇胺和两种未知的磷脂组成。主要的细胞脂肪酸为 iso-C(16：0)、iso-C(15：0)和 C(17：1)ω8c。基因组 DNA 的 G+C 含量为 72.8mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CPCC 201356(T)属于地霉科，与 Geodermatophilus obscurus DSM 43160(T)形成一个独特的分支。该菌与 G. obscurus DSM 43160(T)的 16S rRNA 基因序列相似性为 97.7%。菌株 CPCC 201356(T)与 G. obscurus DSM 43160(T)之间的 DNA-DNA 杂交率为 17.4%。基于多相分类学研究的证据，提出了一个新的种，即红色地霉 Geodermatophilus ruber sp. nov.，其模式菌株为 CPCC 201356(T)（=DSM 45317(T)=CCM 7619(T)）。
