Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany,
Curr Microbiol. 2014 Mar;68(3):365-71. doi: 10.1007/s00284-013-0479-2. Epub 2013 Nov 8.
A novel, gram reaction positive aerobic actinobacterium, designated G12(T), not validly named as Geodermatophilus obscurus subsp. amargosae, was accessed in the DSMZ open collection as DSM 46136(T). The optimal growth was at 25-35 °C, [corrected] at pH 6.0-12.0 and in the absence of NaCl, forming greenish-black-coloured colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G+C content of the strain was 73.0 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a small amount of phosphatidylglycerol; MK-9(H₄) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C₁₅:₀. The 16S rRNA gene showed 94.2-99.5 % sequence identity with the members of the genus Geodermatophilus. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, strain G12(T) is proposed to represent a novel species, Geodermatophilus amargosae. Type strain is G12(T) [=G96] (=DSM 46136(T) = CCUG 62971(T) = MTCC 11559(T) = ATCC 25081(T) = JCM 3153(T) = NBRC 13316(T) = NRRL B-3578(T) = KCTC 9360(T)). The INSDC accession number is HF679056.
一株新型革兰氏阳性需氧放线菌，被命名为 G12(T)，未正式命名为暗褐地霉亚种，是在 DSMZ 公开收藏中作为 DSM 46136(T)被获取的。最适生长温度为 25-35°C，[校正]pH 值为 6.0-12.0，且在无 NaCl 的情况下，在 GYM 琼脂上形成绿黑色菌落。该分离株的化学分类和分子特征与暗褐地霉属的成员相符。该菌株的 DNA G+C 含量为 73.0 mol%。肽聚糖含有 meso-二氨基庚二酸作为诊断二氨基酸。主要磷脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和少量磷脂酰甘油；MK-9(H₄)是主要的menaquinone，检测到半乳糖作为诊断糖。主要细胞脂肪酸为支链饱和酸 iso-C₁₅:₀。16S rRNA 基因与暗褐地霉属的成员表现出 94.2-99.5%的序列同一性。基于化学分类学结果和 16S rRNA 基因序列分析，菌株 G12(T)被提议代表一个新种，暗褐地霉。模式株为 G12(T)[=G96] (=DSM 46136(T) = CCUG 62971(T) = MTCC 11559(T) = ATCC 25081(T) = JCM 3153(T) = NBRC 13316(T) = NRRL B-3578(T) = KCTC 9360(T))。INSDC 登录号为 HF679056。
