Ten Leonid N, Liu Qing-Mei, Im Wan-Taek, Lee Myungjin, Yang Deok-Chun, Lee Sung-Taik
National University of Uzbekistan, Students town, Tashkent, 700-174, Uzbekistan.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2565-2570. doi: 10.1099/ijs.0.64414-0.
A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 104T, was isolated from a soil sample from a ginseng field in Pocheon Province (South Korea) and was characterized taxonomically by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 104T was shown to belong to the family Sphingobacteriaceae, being related to Pedobacter africanus DSM 12126T (97.0%), Pedobacter caeni LMG 22862T (96.9%), Pedobacter cryoconitis DSM 14825T (96.8%) and Pedobacter heparinus DSM 2366T (96.6%). The phylogenetic distance from any other Pedobacter species with a validly published name was greater than 3.4% (i.e.<96.6% 16S rRNA gene sequence similarity). DNA-DNA hybridization experiments showed that values for DNA-DNA relatedness between strain Gsoil 104T and its phylogenetically closest neighbours were below 37%. The G+C content of the genomic DNA was 43.6 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were C16:1omega7c, iso-C15:0, C16:0, iso-C17:0 3-OH and iso-C15:0 2-OH. These chemotaxonomic data support the affiliation of strain Gsoil 104T to the genus Pedobacter. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 104T represents a novel species in the genus Pedobacter, for which the name Pedobacter ginsengisoli sp. nov. is proposed. The type strain is Gsoil 104T (=KCTC 12576T=LMG 23399T).
从韩国抱川市人参田土壤样品中分离出一株革兰氏阴性、严格需氧、杆状、无运动性、不产芽孢的细菌菌株，命名为Gsoil 104T，并采用多相分类法对其进行分类学特征研究。基于16S rRNA基因序列相似性，菌株Gsoil 104T被证明属于鞘氨醇杆菌科，与非洲 Pedobacter africanus DSM 12126T（97.0%）、土壤 Pedobacter caeni LMG 22862T（96.9%）、嗜冷 Pedobacter cryoconitis DSM 14825T（96.8%）和肝素 Pedobacter heparinus DSM 2366T（96.6%）相关。与其他任何有效发表名称的 Pedobacter 物种的系统发育距离均大于3.4%（即16S rRNA基因序列相似性<96.6%）。DNA-DNA杂交实验表明，菌株Gsoil 104T与其系统发育关系最密切的近缘菌株之间的DNA-DNA相关性值低于37%。基因组DNA的G+C含量为43.6 mol%。主要呼吸醌为MK-7。主要脂肪酸为C16:1ω7c、异C15:0、C16:0、异C17:0 3-OH和异C15:0 2-OH。这些化学分类学数据支持菌株Gsoil 104T属于 Pedobacter 属。基于其表型特征和系统发育独特性，菌株Gsoil 104T代表 Pedobacter 属中的一个新物种，为此提出名为 Pedobacter ginsengisoli sp. nov. 的名称。模式菌株为Gsoil 104T（=KCTC 12576T=LMG 23399T）。
