Choi Kang Duk, Siddiqi Muhammad Zubair, Liu Qingmei, Jo Jung Hun, Chun Se Yoon, Choi Gyu-Min, Kim Sang Young, Lee Soon Youl, Im Wan-Taek
Sellusone Co., Ltd., 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
Int J Syst Evol Microbiol. 2018 May;68(5):1436-1441. doi: 10.1099/ijsem.0.002669. Epub 2018 Apr 5.
A Gram-reaction-negative, strictly aerobic, milky-white and rod-shaped bacterium (designated Gsoil 115) isolated from ginseng field soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 115 grew optimally at 30 °C and at pH 7.0 on Reasoner's 2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gsoil 115 belongs to the genus Polaromonas and was most closely related to Polaromonaseurypsychrophila B717-2 (98.6 %), Polaromonasvacuolata 34-P (98.3 %), Polaromonasjejuensis NBRC 106434 (98.1 %), Polaromonas aquatic CCUG 39402 (97.7 %) and Polaromonascryoconiti Cr4-35 (97.5 %). The DNA G+C content was 60.9 mol%. The DNA-DNA hybridization relatedness between strain Gsoil 115 and P. eurypsychrophila B717-2, P. vacuolata 34-P, P. jejuensis NBRC 106434, P. aquatic CCUG 39402 and P. cryoconiti Cr4-35 were 31.2, 21.6, 16.9, 8.7 and 10.1 %, respectively. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The sole respiratory quinone was Q-8. The major fatty acids were C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), which supported the affiliation of strain Gsoil 115 to the genus Polaromonas. Moreover, the physiological, biochemical and low level of DNA-DNA relatedness value allowed the phenotypic and genotypic differentiation of strain Gsoil 115 from the recognized species of the genus Polaromonas. Therefore, strain Gsoil 115 represents a novel species of the genus Polaromonas, for which the name Polaromonas ginsengisoli sp. nov. is proposed, with the type strain Gsoil 115 (LMG 23393=KCTC 12577).
从人参田土壤中分离出的一株革兰氏阴性、严格需氧、乳白色杆状细菌（命名为Gsoil 115），采用多相分类法对其进行表征，以明确其分类地位。菌株Gsoil 115在30 °C和pH 7.0条件下于Reasoner's 2A琼脂培养基上生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株Gsoil 115属于嗜冷单胞菌属，与嗜冷嗜冷单胞菌B717-2（98.6 %）、液泡嗜冷单胞菌34-P（98.3 %）、济州嗜冷单胞菌NBRC 106434（98.1 %）、水生嗜冷单胞菌CCUG 39402（97.7 %）和冰冻嗜冷单胞菌Cr4-35（97.5 %）关系最为密切。DNA G+C含量为60.9 mol%。菌株Gsoil 115与嗜冷嗜冷单胞菌B717-2、液泡嗜冷单胞菌34-P、济州嗜冷单胞菌NBRC 106434、水生嗜冷单胞菌CCUG 39402和冰冻嗜冷单胞菌Cr4-35之间的DNA-DNA杂交相关性分别为31.2、21.6、16.9、8.7和10.1 %。主要极性脂类为磷脂酰甘油（PG）、二磷脂酰甘油（DPG）和磷脂酰乙醇胺（PE）。唯一的呼吸醌为Q-8。主要脂肪酸为C16 : 0和总和特征3（C16 : 1ω7c和/或C16 : 1ω6c），这支持了菌株Gsoil 115属于嗜冷单胞菌属。此外，生理生化特征以及低水平的DNA-DNA相关性值使得菌株Gsoil 115能够与嗜冷单胞菌属中已确认的物种在表型和基因型上区分开来。因此，菌株Gsoil 115代表嗜冷单胞菌属的一个新物种，并提议将其命名为嗜冷人参单胞菌（Polaromonas ginsengisoli sp. nov.），模式菌株为Gsoil 115（LMG 23393=KCTC 12577）。
