Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China.
Med Sci Monit. 2010 Mar;16(3):CR103-108.
Recent studies have shown that elevated plasma interleukin-6 (IL-6) levels in coronary heart disease (CHD) patients are associated with polymorphisms in the promoter region of the IL-6 gene. However, related studies of these phenomenon are rare in the Han population of China. The aim was to develop a rapid IL-6 gene genotyping assay by fluorescent resonance energy transfer (FRET) and melting curves on a LightCycler system and then study the association between IL-6 gene polymorphisms and CHD.
MATERIAL/METHODS: Two hundred thirty-one CHD patients and 210 controls, all of Han ethnicity from northern China, were analyzed by the established method. DNA sequencing analysis confirmed the results.
Three genotypes were found for the -572G/C polymorphism in the Han Chinese. Statistical analysis for the this polymorphism revealed a significant difference between G allele carriers (GG+GC) and non-G allele carriers (CC) in both the CHD and the control group. Ten cases were identified to be of GA genotype for the -597G/A polymorphism in the Han Chinese. PCR product sequencing confirmed all the results.
A rapid IL-6 gene genotyping assay was developed. The clinical data revealed that the -572G/C polymorphism in the IL-6 gene promoter region is involved in the pathogenesis and progression of CHD in the Han Chinese.
最近的研究表明，冠心病（CHD）患者血浆白细胞介素-6（IL-6）水平升高与 IL-6 基因启动子区域的多态性有关。然而，在中国汉族人群中，相关研究较为罕见。本研究旨在建立一种基于荧光共振能量转移（FRET）和熔解曲线的快速 IL-6 基因基因分型检测方法，并探讨 IL-6 基因多态性与 CHD 的关系。
材料/方法：采用建立的方法对 231 例 CHD 患者和 210 例对照者（均为中国北方汉族人）进行分析。DNA 测序分析对结果进行了验证。
在汉族人群中发现了-572G/C 多态性的三种基因型。对该多态性进行统计学分析显示，CHD 组和对照组中 G 等位基因携带者（GG+GC）与非 G 等位基因携带者（CC）之间存在显著差异。在汉族人群中，-597G/A 多态性有 10 例为 GA 基因型。PCR 产物测序证实了所有结果。
建立了一种快速 IL-6 基因基因分型检测方法。临床资料显示，IL-6 基因启动子区-572G/C 多态性与汉族人群 CHD 的发病机制和进展有关。
