橙花叔醇及其潜在抗炎活性的研究。

Terpenoids from Daphne aurantiaca and their potential anti-inflammatory activity.

机构信息

School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, People's Republic of China.

出版信息

J Nat Prod. 2010 Apr 23;73(4):532-5. doi: 10.1021/np9005053.

PMID:20192236

Abstract

Phytochemical examination of the methanolic extract from the stem bark of Daphne aurantiaca led to the isolation of six new sesquiterpenoids, dauca-3,11-dien-2alpha,15-diol (1), 3-oxoguai-4-ene-11,12-diol (2), 4alpha,5alpha,8alpha,11alphaH-3-oxoguai-1(10)-en-12,8-olide-7alpha-diol (3), 4alpha,5alpha,8alpha,11betaH-3-oxoguai-1(10)-en-12,8-olide-7beta-diol (4), 4alpha,5betaH-guai-9,7(11)-dien-12,8-olide-1alpha,8alpha-diol (5), 4alpha,5alphaH-guai-9,7(11)-dien-12,8-olide-1alpha,8alpha-diol (6), and a new diterpenoid, 12-O-benzoylphorbol 13-nonanoate (7), together with 10 known terpenoids. All compounds were tested for inhibitory activity against LPS-induced NO production in RAW 264.7 macrophages. Compounds 7, 8, 9, 10, and 11 showed potent inhibitory activities against NO production with IC(50) values of 0.01, 0.01, 0.06, 0.07, and 0.03 microM, respectively.

摘要

从山胡椒科杜茎山属植物杜茎山（Daphne aurantiaca）的茎皮甲醇提取物中分离得到 6 个新的倍半萜类化合物，分别为：达卡-3,11-二烯-2α,15-二醇（1）、3-氧代-11,12-二羟基-4-表-愈创木烯（2）、4α,5α,8α,11αH-3-氧代-1(10)-桉叶-12,8-内酯-7α-二醇（3）、4α,5α,8α,11βH-3-氧代-1(10)-桉叶-12,8-内酯-7β-二醇（4）、4α,5βH-愈创木-9,7(11)-二烯-12,8-内酯-1α,8α-二醇（5）、4α,5αH-愈创木-9,7(11)-二烯-12,8-内酯-1α,8α-二醇（6）和一个新的二萜类化合物，12-O-苯甲酰佛波醇 13-壬酸酯（7），以及 10 个已知的萜类化合物。所有化合物均测试了对 RAW 264.7 巨噬细胞中 LPS 诱导的 NO 产生的抑制活性。化合物 7、8、9、10 和 11 对 NO 产生表现出很强的抑制活性，IC50 值分别为 0.01、0.01、0.06、0.07 和 0.03 μM。