Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
BMB Rep. 2010 Feb;43(2):122-6. doi: 10.5483/bmbrep.2010.43.2.122.
Homozygous staggerer (RORa(sg/sg)) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However, there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus, thalamus, cortex, and olfactory bulb. The expressions of Ki67, doublecortin (DCX), and NeuN, which are cell proliferation, neuronal differentiation and mature neuron markers, respectively, were measured with immunohistochemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.
纯合 staggerer（RORa(sg/sg)）小鼠表现出严重的共济失调，这是由小脑退化引起的。减少和功能失调的 Rora 是这种神经表型的主要原因。 staggerer 小鼠的表型在小脑方面已广为人知。然而，尽管 staggerer 仅在小脑但在海马体、丘脑、皮层和嗅球中表达，但很少有关于大脑的报道。 staggerer 小鼠的齿状回中细胞增殖、神经元分化和成熟神经元标志物 Ki67、双皮质素（DCX）和 NeuN 的表达分别通过免疫组织化学进行了测量，以揭示 staggerer 是否会影响齿状回的变化。 staggerer 小鼠齿状回的 DCX 和 NeuN 免疫反应性明显低于正常对照组，而 Ki67 在 staggerer 小鼠中很少变化。这些结果表明， staggerer 突变不仅在小脑，而且在齿状回中对神经元分化和发育有影响。
