Xu Xue-bin, Yuan Zheng-an, Jin Hui-ming, Xiao Wen-jia, Gu Bao-ke, Chen Min, Ran Lu, Diao Bao-wei, Cui Zhi-gang, Hu Qing-hua, Kan Biao
Shanghai Center of Disease Control and Prevention, Shanghai 200336, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Sep;30(9):933-7.
To study the molecular epidemiological characteristics of Salmonella enterica subsp. enterica serovar Senftenberg (Salmonella senftenberg) in Shanghai, from 2006 to 2007.
A retrospective analysis in 2006 and 2007 was performed to explore the source of food-borne Salmonella senftenberg. The isolates from diarrhea patients between 2006 and 2007 were identified, including biochemical test, hilA and invA gene phenotyping, drug susceptibility, Riboprinter((R)) (RP) and pulsed-field gel electrophoresis (PFGE).
Of the diarrhea patients isolates in the monitoring program on non-typhi Salmonella infection in the year of 2006 in Shanghai, number of patients caused by Salmonella senftenberg ranked the third. The stock of Salmonella senftenberg food-born isolates were derived from swine and beef products between 2003 and 2005. All of the strains from diarrhea patients were susceptible to antibiotics except tetracylina (75.6%). With RP and PFGE molecular typing, the two groups (with hydrogen sulfide and hilA, invA gene or without) could be divided into two different independent clone cluster in genetics. 34 strains of diarrhea were divided into 16 PFGE typing-pattern, and among them 12 strains including type 4 (4 strains), type 5 (1 strains), type 6 (6 strains), type 7 (1 strains) and 13 strains including type 11 (3 strains), type 17 (5 strains), type 23 (5 strains) were two different dominant clone cluster.
The epidemic of Salmonella senftenberg within 2006 might have been the result of a long period of case occurrence in Shanghai. This rare outbreak belonged to a cluster of outbreaks caused by two different PFGE clone clusters. Data suggested that the genetic clone of Salmonella senftenberg might have been unstable and the source of contamination were complicated, with the characteristics as the obvious decreasing number of patients, with no food-borne isolates in 2007.
研究2006年至2007年上海市肠炎沙门氏菌肠炎亚种森夫滕贝格血清型（肠炎沙门氏菌森夫滕贝格）的分子流行病学特征。
对2006年和2007年进行回顾性分析，以探究食源性肠炎沙门氏菌森夫滕贝格的来源。对2006年至2007年腹泻患者的分离株进行鉴定，包括生化试验、hilA和invA基因表型分析、药敏试验、核糖体分型（RP）和脉冲场凝胶电泳（PFGE）。
在2006年上海市非伤寒沙门氏菌感染监测项目中腹泻患者的分离株中，肠炎沙门氏菌森夫滕贝格引起的患者数量排名第三。2003年至2005年食源性肠炎沙门氏菌森夫滕贝格分离株的来源是猪和牛肉产品。除四环素（75.6%）外，所有腹泻患者分离株对其他抗生素均敏感。通过RP和PFGE分子分型，两组（有或无硫化氢及hilA、invA基因）在遗传学上可分为两个不同的独立克隆簇。34株腹泻菌株分为16种PFGE分型模式，其中4型（4株）、5型（1株）、6型（6株）、7型（1株）共12株和11型（3株）、17型（5株）、23型（5株）共13株为两个不同的优势克隆簇。
2006年肠炎沙门氏菌森夫滕贝格的流行可能是上海市长期病例发生的结果。此次罕见疫情属于由两个不同PFGE克隆簇引起的聚集性疫情。数据表明肠炎沙门氏菌森夫滕贝格的基因克隆可能不稳定，污染来源复杂，具有患者数量明显减少的特点，2007年无食源性分离株。
