[缺失沙门氏菌致病岛1的肠炎沙门氏菌森夫滕贝格血清型的特征]
[Characteristics of Salmonella enterica serovar Senftenberg lacking Salmonella pathogenicity island 1].
作者信息
Li Ying-hui, Xu Xue-bin, Hu Qing-hua, Shi Xiao-lu, Lin Yi-man, Qiu Ya-qun
机构信息
Microbiology Department of Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2011 Oct;45(10):899-903.
OBJECTIVE
To study the characteristics of the strains of Salmonella enterica (S. enterica) serovar Senftenberg lacking Salmonella pathogenicity island 1 (SPI-1).
METHODS
A total of 10 strains of S. enterica serovar Senftenberg were isolated from 10 cases of diarrhea patients. Pulsed field gel electrophoresis (PFGE), PCR, sequencing techniques and cell invasion test were adapted to study the molecular types and invasiveness of the genes and cells; and made a comparison between the 10 strains and the strains (C02013) isolated in Shenzhen in 2002.
RESULTS
The 10 Senftenberg isolated (S09007-S09012, S09014-S09017) in Shanghai showed three PFGE patterns, which were significantly different from the strains isolated in Shenzhen. PCR-amplified results indicated the invasion gene (invA), secreted effector protein gene (sipA) and gene fragments as fhlA-hilA, hilA-spaP and spaP-invH in the 10 strains of SPI-1 were all negative. The sequencing results revealed that the 10 strains isolated in Shanghai lacked most parts of SPI-1 genes, as fragments from orgA to invH and parts of orgA gene itself; however, compared with strains isolated in Shenzhen, the sprB-orgC gene existed. The missing parts of genes were replaced by a simple insertion sequence (IS) of 1000 bp in the strains isolated both in Shenzhen in 2002 and in Shanghai in 2006. The invasiveness rates of the 10 strains (S09007-S09012, S09014-S09017) towards Hela cells were (0.0053 ± 0.0024)%, (0.0046 ± 0.0006)%, (0.0047 ± 0.0003)%, (0.0064 ± 0.0012)%, (0.0065 ± 0.0011)%, (0.0070 ± 0.0020)%, (0.0115 ± 0.0030)%, (0.0099 ± 0.0039)%, (0.0180 ± 0.0135)% and (0.0031 ± 0.0012)%, respectively; which were all significantly lower than the rate of invA-positive control strain STM1344 ((5.0800 ± 0.6333)%); lower or close to the rate of invA-lacked artificial-mutated strain STMinvA-((0.0193 ± 0.0045)%).
CONCLUSION
SPI-1 genes are not essential for the diarrhea caused by S. enterica serovar Senftenberg.
目的
研究缺失沙门氏菌致病岛1（SPI-1）的肠炎沙门氏菌（S. enterica）森夫滕贝格血清型菌株的特征。
方法
从10例腹泻患者中分离出10株肠炎沙门氏菌森夫滕贝格血清型菌株。采用脉冲场凝胶电泳（PFGE）、聚合酶链反应（PCR）、测序技术及细胞侵袭试验，研究这些菌株的分子类型及基因与细胞的侵袭性，并将这10株菌株与2002年在深圳分离出的菌株（C02013）进行比较。
结果
在上海分离出的10株森夫滕贝格菌株（S09007-S09012，S09014-S09017）呈现出三种PFGE图谱，与在深圳分离出的菌株有显著差异。PCR扩增结果表明，这10株SPI-1菌株中的侵袭基因（invA）、分泌效应蛋白基因（sipA）以及fhlA-hilA、hilA-spaP和spaP-invH基因片段均为阴性。测序结果显示，在上海分离出的10株菌株缺失SPI-1基因的大部分区域，即从orgA到invH的片段以及orgA基因本身的部分区域；然而，与在深圳分离出的菌株相比，sprB-orgC基因存在。2002年在深圳及2006年在上海分离出的菌株中，缺失的基因部分被一个1000 bp的简单插入序列（IS）所取代。这10株菌株（S09007-S09012，S09014-S09017）对Hela细胞的侵袭率分别为（0.0053±0.0024）%、（0.0046±0.0006）%、（0.0047±0.0003）%、（0.0064±0.0012）%、（0.0065±0.0011）%、（0.0070±0.0020）%、（0.0115±0.0030）%、（0.0099±0.0039）%、（0.0180±0.0135）%和（0.003
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