Rabsch W, Prager R, Koch J, Stark K, Roggentin P, Bockemühl J, Beckmann G, Stark R, Siegl W, Ammon A, Tschäpe H
National Reference Centre for Salmonella and other Enteric Pathogens, Robert Koch Institute, Wernigerode, Germany.
Epidemiol Infect. 2005 Oct;133(5):837-44. doi: 10.1017/S0950268805004152.
During 2002-2003 increased numbers of notified salmonellosis due to S. enterica serovar Agona were observed in Germany. In order to understand the recent spread of this serovar and to trace the route of infection to its source, a new phage-typing scheme and pulsed field gel electrophoresis (PFGE) were used to analyse these isolates. By using 14 bacteriophages, 52 phage types were distinguished among the S. Agona strains. PFGE also differentiated 52 different patterns. A combination of both methods generated 94 clonal types among 165 S. Agona strains originating from Germany and other countries including the United States, United Arab Emirates, Turkey, India, Austria and Finland, indicating a great biological diversity within this serovar. However, 36 recent S. Agona isolates from infantile gastroenteritis in Germany, from an untreated batch of aniseed imported from Turkey and from fennel-aniseed-caraway infusion (packed in tea bags) revealed clonal identity indicating their epidemiological relatedness as a new source of infection. It is suggested that strains of S. Agona will continue to be of public health concern, and that phage typing together with PFGE typing should be applied as reliable and rapid tools for epidemiological subtyping and future monitoring.
2002年至2003年期间，德国报告的肠炎沙门氏菌阿贡纳血清型引起的沙门氏菌病病例数有所增加。为了解该血清型近期的传播情况并追踪感染源，采用了一种新的噬菌体分型方案和脉冲场凝胶电泳（PFGE）技术对这些分离株进行分析。通过使用14种噬菌体，在阿贡纳沙门氏菌菌株中区分出52种噬菌体类型。PFGE也区分出52种不同的图谱。两种方法结合在来自德国以及包括美国、阿拉伯联合酋长国、土耳其、印度、奥地利和芬兰等其他国家的165株阿贡纳沙门氏菌菌株中产生了94种克隆型，表明该血清型具有很大的生物多样性。然而，最近从德国婴儿肠胃炎、从一批未经处理的从土耳其进口的茴芹以及从茴香-茴芹-葛缕子浸液（装在茶包中）分离出的36株阿贡纳沙门氏菌显示出克隆一致性，表明它们作为新的感染源在流行病学上具有相关性。有人认为，阿贡纳沙门氏菌菌株仍将是公共卫生关注的对象，噬菌体分型与PFGE分型应作为可靠且快速的工具用于流行病学亚型分析和未来监测。
