Istituto Italiano di Tecnologia, Nanophysics Department, I-16163, Genova, Italy.
IEEE Trans Nanobioscience. 2013 Jun;12(2):106-11. doi: 10.1109/TNB.2013.2257835.
We have fabricated nanoporous alumina surfaces by means of anodization in oxalic acid in different conditions and used them as the substrates for the growth of cells from a human osteoblast-like cell line. The rough nanoporous alumina substrates have been compared both with smooth standard Petri dishes used as the control and with commercial substrates of similar material. The viability of the cells has been assessed at different culture times of 4, 11, 18, and 25 days in vitro. It turned out that the porous side of the galvanostatically fabricated alumina performed similar to the control and better than the commercial porous alumina, whereas the potentiostatically fabricated porous alumina performed better than all the other substrates at all times, and in particular at the two shortest time periods of 4 and 11 days in vitro. The best performance of the substrates is associated with intermediate surface roughness and feature spacing.
我们通过在不同条件下的草酸阳极氧化法制造了纳米多孔氧化铝表面，并将其用作人成骨样细胞系细胞生长的基底。粗糙的纳米多孔氧化铝基底与用作对照的光滑标准培养皿以及类似材料的商业基底进行了比较。在体外培养 4、11、18 和 25 天的不同时间点评估了细胞的活力。结果表明，恒电流法制备的多孔氧化铝的多孔侧与对照相似，优于商业多孔氧化铝，而恒电位法制备的多孔氧化铝在所有时间点均优于所有其他基底，特别是在体外培养的 4 天和 11 天这两个最短时间点。基底的最佳性能与中等表面粗糙度和特征间距有关。
