Taylor P C
Department of Microbiology, Prince of Wales Hospital, Randwick, New South Wales, Australia.
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):249-52. doi: 10.1007/BF01974545.
This study examines the application in a routine laboratory of a method for concentrating and lysing leucocytes in continuous ambulatory peritoneal dialysis (CAPD) effluent to diagnose peritonitis. Between November 1986 and April 1990 pathogens from 42 of 82 (51%) CAPD specimens from patients with acute peritonitis failed to grow on horse blood agar (HBA) after direct inoculation. Recovery rates of pathogens were 74% (6 of 82) from a solid medium containing saponin and 89% (73 of 82) after lysing the leucocytes before inoculating HBA. Microbial sequestration in leucocytes was demonstrated in 73% (60 of 82) of specimens, while pathogens from 11% (9 of 82) were grown only from a liquid medium. Thirteen of 17 specimens with < 500 leucocytes/mm3 grew on all three solid media. Gram stain microscopy revealed organisms in 51% (41 of 81) of specimens. The method, using apparatus and media prepared for everyday examination of specimens, is sensitive, specific and yields single colonies of most species within 24 h.
本研究探讨了一种用于浓缩和裂解持续性非卧床腹膜透析（CAPD）流出液中白细胞以诊断腹膜炎的方法在常规实验室中的应用。1986年11月至1990年4月期间，82例急性腹膜炎患者的CAPD标本中有42例（51%）的病原体在直接接种后未能在马血琼脂（HBA）上生长。在含有皂苷的固体培养基中病原体的回收率为74%（82例中的6例），在接种HBA前裂解白细胞后回收率为89%（82例中的73例）。82例标本中有73%（60例）显示白细胞中有微生物滞留，而11%（82例中的9例）的病原体仅从液体培养基中生长。17例白细胞计数<500/mm³的标本中有13例在所有三种固体培养基上均生长。革兰氏染色显微镜检查显示81例标本中有51%（41例）存在微生物。该方法使用为日常标本检查准备的仪器和培养基，具有敏感性、特异性，且能在24小时内培养出大多数菌种的单菌落。
