Weenk G, Fitzmaurice E, Mossel D A
Nutricia Research, Microbiological Research Laboratory, Zoetermeer, The Netherlands.
J Appl Bacteriol. 1991 Feb;70(2):135-43. doi: 10.1111/j.1365-2672.1991.tb04439.x.
The suitability of a variety of media and procedures for the enumeration of sulphite-reducing clostridia in food was investigated. The most suitable procedure was pasteurization of the 1/10 macerate for at least 1 min at 80 degrees C; followed by culture at 30 degrees C for up to 3 d in a sulphite-based, differential reinforced clostridium medium, without bicarbonate or lactate but with an increased iron concentration, and sulphite and iron added after sterilization. Black sulphite-reducing colonies were finally tested for sensitivity to metronidazole and confirmation of their failure to grow on agar slopes under aerobic conditions.
研究了多种培养基和程序用于食品中还原亚硫酸盐梭状芽孢杆菌计数的适用性。最合适的程序是将1/10浸软物在80℃下至少巴氏灭菌1分钟；然后在30℃下于不含碳酸氢盐或乳酸盐但铁浓度增加的亚硫酸盐基鉴别强化梭状芽孢杆菌培养基中培养长达3天，亚硫酸盐和铁在灭菌后添加。最后对黑色的还原亚硫酸盐菌落进行甲硝唑敏感性测试，并确认它们在有氧条件下不能在琼脂斜面上生长。
