de Jong A E I, Eijhusen G P, Brouwer-Post E J F, Grand M, Johansson T, Kärkkäinen T, Marugg J, in't Veld P H, Warmerdam F H M, Wörner G, Zicavo A, Rombouts F M, Beumer R R
Food Hygiene and Microbiology, Wageningen University, Wageningen, The Netherlands.
J Microbiol Methods. 2003 Sep;54(3):359-66. doi: 10.1016/s0167-7012(03)00069-1.
Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens (SFP) agar, sulfite cycloserine azide (SCA), differential clostridial agar (DCA), and oleandomycin polymyxin sulfadiazine perfringens (OPSP) agar] were compared in a prestudy, of which four (IS, TSC, SCA, and DCA) were selected for an international collaborative trial. Recovery of 15 pure strains was tested in the prestudy and recovery of one strain from foodstuffs was tested in the collaborative trial. Results from the prestudy did reveal statistical difference of the media but recoveries on all media were within the microbiological limits (+/-30%) of IS, which was set as a reference medium. Recoveries on the media tested in the collaborative trial were statistically different as well, but these differences were of no microbiological-analytical relevance. Food matrices did not affect the recovery of C. perfringens in general. DCA and SCA, in particular, are labor-intensive to prepare and DCA frequently failed to produce black colonies; gray colonies were quite common. Since IS medium is nonselective, it was concluded that TSC was the most favorable medium for the enumeration of C. perfringens from foods.
已开发出多种培养基用于从食品中计数产气荚膜梭菌。在本研究中，在一项预试验中比较了六种培养基[亚硫酸铁（IS）琼脂、胰蛋白胨亚硫酸盐环丝氨酸（TSC）琼脂、沙希迪弗格森产气荚膜梭菌（SFP）琼脂、亚硫酸盐环丝氨酸叠氮化物（SCA）、鉴别梭菌琼脂（DCA）和竹桃霉素多粘菌素磺胺嘧啶产气荚膜梭菌（OPSP）琼脂]，其中四种（IS、TSC、SCA和DCA）被选用于一项国际协作试验。在预试验中测试了15株纯菌株的回收率，在协作试验中测试了从食品中回收1株菌株的情况。预试验结果确实显示了培养基之间的统计学差异，但所有培养基上的回收率都在作为参考培养基的IS的微生物学限度（±30%）之内。协作试验中测试的培养基上的回收率也存在统计学差异，但这些差异在微生物分析方面并无相关性。一般来说，食品基质不会影响产气荚膜梭菌的回收率。特别是DCA和SCA制备起来劳动强度大，而且DCA经常无法产生黑色菌落；灰色菌落相当常见。由于IS培养基是非选择性的，得出的结论是TSC是从食品中计数产气荚膜梭菌的最适宜培养基。
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