棘皮动物海胆 Paracentrotus lividus 中核糖体成熟过程中的一种新型细胞质加工事件的证据。
Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus.
机构信息
Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Palermo, V.le delle Scienze, Edificio 16, 90128, Palermo, Italy.
出版信息
Cell Mol Life Sci. 2010 Jun;67(11):1871-9. doi: 10.1007/s00018-010-0297-7. Epub 2010 Feb 27.
Abstract
In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling.
摘要
在本文中，我们证明了一个以前从未描述过的细胞质加工步骤的存在，该步骤涉及海胆 Paracentrotus lividus 的核糖体前亚基。Northern 印迹杂交、引物延伸、S1 作图实验和原位杂交实验使我们能够证明细胞质加工颗粒被相继重新导入细胞核，在被运送到细胞质之前，它们的 RNA 在细胞核中完成成熟过程。我们的研究结果提出了一个新的核糖体成熟和穿梭模型。
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本文引用的文献
1
The role of human ribosomal proteins in the maturation of rRNA and ribosome production.人类核糖体蛋白在核糖体RNA成熟和核糖体产生过程中的作用。
RNA. 2008 Sep;14(9):1918-29. doi: 10.1261/rna.1132008. Epub 2008 Aug 12.
2
The post-transcriptional steps of eukaryotic ribosome biogenesis.真核生物核糖体生物发生的转录后步骤。
Cell Mol Life Sci. 2008 Aug;65(15):2334-59. doi: 10.1007/s00018-008-8027-0.
3
Nuclear export and cytoplasmic maturation of ribosomal subunits.核糖体亚基的核输出与细胞质成熟
FEBS Lett. 2007 Jun 19;581(15):2783-93. doi: 10.1016/j.febslet.2007.05.013. Epub 2007 May 11.
4
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels.蛋白质和DNA在水平十二烷基硫酸钠聚丙烯酰胺凝胶上的电泳。
Immun Ageing. 2006 Jul 12;3:7. doi: 10.1186/1742-4933-3-7.
5
Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae.酿酒酵母核仁亚区域内核限制前核糖体的监测。
EMBO J. 2006 Apr 5;25(7):1534-46. doi: 10.1038/sj.emboj.7601035. Epub 2006 Mar 16.
6
An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA.前核糖体RNA上U3小核仁RNA（snoRNA）优先结合位点的进化性分子内转移。
Nucleic Acids Res. 2005 Sep 6;33(15):4995-5005. doi: 10.1093/nar/gki815. Print 2005.
7
Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells.哺乳动物细胞中40S核糖体亚基前体的核输出及胞质加工
EMBO J. 2005 Aug 17;24(16):2862-72. doi: 10.1038/sj.emboj.7600752. Epub 2005 Jul 28.
8
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Curr Opin Cell Biol. 2005 Jun;17(3):281-6. doi: 10.1016/j.ceb.2005.04.001.
9
The nucleolus and transcription of ribosomal genes.核仁与核糖体基因的转录。
Biol Cell. 2004 Oct;96(8):579-94. doi: 10.1016/j.biolcel.2004.04.015.
10
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Mol Cell. 2004 Jul 2;15(1):17-29. doi: 10.1016/j.molcel.2004.05.020.
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