棘皮动物海胆 Paracentrotus lividus 中核糖体成熟过程中的一种新型细胞质加工事件的证据。
Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus.
机构信息
Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Palermo, V.le delle Scienze, Edificio 16, 90128, Palermo, Italy.
出版信息
Cell Mol Life Sci. 2010 Jun;67(11):1871-9. doi: 10.1007/s00018-010-0297-7. Epub 2010 Feb 27.
Abstract
In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling.
摘要
在本文中，我们证明了一个以前从未描述过的细胞质加工步骤的存在，该步骤涉及海胆 Paracentrotus lividus 的核糖体前亚基。Northern 印迹杂交、引物延伸、S1 作图实验和原位杂交实验使我们能够证明细胞质加工颗粒被相继重新导入细胞核，在被运送到细胞质之前，它们的 RNA 在细胞核中完成成熟过程。我们的研究结果提出了一个新的核糖体成熟和穿梭模型。
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