Cantone M, Barbieri R, Duro G, Izzo V, Giudice G
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Mol Biol Rep. 1993 Oct;18(3):177-82. doi: 10.1007/BF01674428.
We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor while has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.
我们已经从海胆Paracentrotus lividus中分离并测序了一个基因间隔区以及rRNA编码基因(rDNA)侧翼区域的一小部分(18S和26S rRNA编码区)。该区域长度约为3.8 Kb。Northern印迹杂交和S1图谱实验表明,在rRNA合成速率较低的发育阶段,也存在一种部分加工的21S rRNA前体,其5'末端与32S初级前体相同。