Mitchell W J, Summers B A, Appel M J
J. A. Baker Institute for Animal Health, Department of Microbiology, Cornell University, Ithaca, NY.
J Comp Pathol. 1991 Jan;104(1):77-87. doi: 10.1016/s0021-9975(08)80090-4.
We have characterized the relationship between the expression of canine distemper virus (CDV) and demyelinating lesions in the white matter of the cerebellum of experimentally infected dogs. In animals which had demyelinating lesions, CDV proteins (N, P, F and H) were expressed and infectious virus could be recovered from brain tissue. Viral proteins (N, P, F and H) were detected by monoclonal antibodies and immunocytochemistry within demyelinating lesions as well as in scattered glial cells in areas of the white matter which lacked detectable lesions. Many cell types, including astrocytes, neurons, ependymal cells, choroid plexus cells, meningeal cells and perivascular inflammatory cells were labelled for viral antigen. We conclude from our results that the mechanism of demyelination in canine distemper virus-induced encephalitis involves expression of viral gene products at the lesion site.
我们已经描述了犬瘟热病毒（CDV）的表达与实验感染犬小脑白质脱髓鞘病变之间的关系。在出现脱髓鞘病变的动物中，CDV蛋白（N、P、F和H）得以表达，并且可从脑组织中分离出传染性病毒。通过单克隆抗体和免疫细胞化学方法，在脱髓鞘病变以及白质中缺乏可检测病变区域的散在神经胶质细胞内检测到病毒蛋白（N、P、F和H）。包括星形胶质细胞、神经元、室管膜细胞、脉络丛细胞、脑膜细胞和血管周围炎性细胞在内的多种细胞类型都被标记上了病毒抗原。我们从结果中得出结论，犬瘟热病毒诱导的脑炎中脱髓鞘机制涉及病变部位病毒基因产物的表达。
