Plant Chemetics Lab, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829 Cologne, Germany.
Curr Opin Biotechnol. 2010 Apr;21(2):225-33. doi: 10.1016/j.copbio.2010.02.003. Epub 2010 Mar 1.
Protein activity is essential functional information, yet difficult to predict from transcript or protein data. Activity-based protein profiling (ABPP) displays active proteins in proteomes using small molecule probes that irreversibly label proteins in their active state. Here, we review proof-of-concept ABPP studies in plant science. These studies displayed activities of dozens of plant cysteine proteases, lipases, methylesterases and the proteasome. ABPP in plants revealed differential protein activities in development and immunity and uncovered striking selectivity of pathogen-derived inhibitors and unexpected targets of commercial inhibitors. The unique, high-content information of ABPP and the robustness and simplicity of the assays will make ABPP a powerful tool in future plant science and biotechnology.
蛋白质活性是至关重要的功能信息，但难以从转录本或蛋白质数据中预测。基于活性的蛋白质分析（ABPP）使用小分子探针在蛋白质组中显示活性蛋白质，这些探针不可逆地标记活性状态下的蛋白质。在这里，我们回顾了植物科学中的概念验证 ABPP 研究。这些研究显示了数十种植物半胱氨酸蛋白酶、脂肪酶、甲酯酶和蛋白酶体的活性。植物中的 ABPP 揭示了发育和免疫过程中蛋白质活性的差异，并发现了病原体衍生抑制剂的惊人选择性和商业抑制剂的意外靶标。ABPP 的独特、高信息量以及测定方法的稳健性和简单性，将使其成为未来植物科学和生物技术的有力工具。
