Institute of Clinical Medicine and Department of Tropical Medicine, National Yang Ming University, Taipei, Taiwan, ROC.
Int Arch Allergy Immunol. 2010;152(4):368-77. doi: 10.1159/000288290. Epub 2010 Mar 3.
Domestic mites are present in house dust samples throughout the world. Reports have shown a high prevalence of Tyrophagus putrescentiae (Tp) sensitization in Europe and Asia, and its importance and clinical relevance in elderly subjects have grown rapidly. The main objective of this study was to investigate the prevalence of Tp sensitization in elderly subjects in a veterans' nursing home using mite allergen extracts and recombinant allergens.
A total of 199 subjects were enrolled in this study: 112 elderly subjects from a nursing home and 87 healthy young adults from the hospital staff as controls. The prevalence of Tp hypersensitivity was determined by specific IgE measurements and basophil histamine release. Immunoblotting with or without inhibition with Dermatophagoides pteronyssinus (Dp) was performed to identify the major allergens and species-specific allergen to Tp.
It was determined that 39.3% (44/112) of the elderly population were sensitized to Tp and 17.9% (20/112) to Tp alone. There was a significantly higher prevalence of Tp hypersensitivity in elderly subjects in comparison with the young adult population. In the age association study of Tp and Dp sensitization, the elderly subjects were more sensitized to Tp than to Dp (p = 0.02). Among the elderly subjects with chronic obstructive pulmonary disease (COPD) 45.8% (11/24) were Tp sensitive. The major allergens, Tyr p 2 and Tyr p 3, were identified with molecular weights of 16 kDa (53%) and 26 kDa (50%) as determined by ELISA and immunoblot inhibition analyses.
The prevalence of Tp sensitization was higher in elderly subjects, especially in patients with COPD. The high percentage of IgE-binding components to the allergens Tyr p 2 and Tyr p 3 indicated that both allergens may play a role in the pathogenesis of IgE-mediated allergic diseases in elderly populations.
国内的尘螨存在于世界各地的房屋灰尘样本中。有报道显示，在欧洲和亚洲，腐食酪螨（Tp）致敏的发生率很高，其在老年人群中的重要性和临床相关性迅速增加。本研究的主要目的是使用螨过敏原提取物和重组过敏原检测退伍军人疗养院老年人群中腐食酪螨的致敏情况。
本研究共纳入 199 例受试者：疗养院的 112 例老年受试者和医院工作人员的 87 例健康年轻成年人作为对照。通过特异性 IgE 测量和嗜碱性粒细胞组胺释放来确定 Tp 超敏反应的发生率。进行免疫印迹分析，同时或不与屋尘螨（Dp）进行抑制，以鉴定主要过敏原和Tp 的种特异性过敏原。
确定 39.3%（44/112）的老年人群对 Tp 过敏，17.9%（20/112）仅对 Tp 过敏。与年轻成年人相比，老年人群中 Tp 超敏反应的发生率明显更高。在 Tp 和 Dp 致敏的年龄关联研究中，老年受试者对 Tp 的敏感性高于 Dp（p = 0.02）。在患有慢性阻塞性肺疾病（COPD）的老年受试者中，45.8%（11/24）对 Tp 敏感。主要过敏原 Tyr p 2 和 Tyr p 3 通过 ELISA 和免疫印迹抑制分析确定分子量为 16 kDa（53%）和 26 kDa（50%）。
老年受试者中 Tp 致敏的发生率较高，尤其是在 COPD 患者中。对过敏原 Tyr p 2 和 Tyr p 3 的 IgE 结合成分的高百分比表明，这两种过敏原可能在老年人群中 IgE 介导的过敏性疾病的发病机制中发挥作用。
