Yu Ching-Hsiang, Tsai Jaw-Ji, Lin Yi-Hsueh, Yu Sheng-Jie, Liao En-Chih
Department of Medicine, MacKay Medical College, New Taipei City 252, Taiwan.
Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, Asia University Hospital, Taichung 413, Taiwan.
Diagnostics (Basel). 2020 Sep 2;10(9):665. doi: 10.3390/diagnostics10090665.
Mite allergens are considerable factors in the genesis of allergic diseases. The storage mite (Tp) appears in contaminated foods and household surroundings. The current diagnostic tools for Tp allergy are mostly based on crude extracts and still contain shortcomings. This study aimed to investigate the immunoglobulin E (IgE)- responsiveness profiles of Tp-allergic patients and develop a molecular diagnostic method using recombinant allergens. Allergenic components were characterized as cross-reacting or species-specific allergens, in which the effective combinations of recombinant allergens were developed and analyzed in terms of the prediction accuracy for clinical diagnosis. Seven recombinant allergens were cloned and generated to detect the IgE responsiveness of the Tp allergy. A survey on the prevalence of mite allergy showed there were higher sensitizations with IgE responsiveness to house dust mites (HDM) (78.9-80.9%) than to storage mites Tp (35.6%). Prevalence of sensitization to Tp was higher in elderly subjects. The principal IgE-binding components of Tp were Tyr p 1, Tyr p 2 and Tyr p 3. Prediction accuracy for Tp allergy by IgE-responsiveness combination D (Tyr p 1, Tyr p 2 & Tyr p 3) was with high precision (100%). Avoiding the cross-reactivity of , the prediction accuracy of IgE-responsiveness combination H+ (Tyr p 1, Tyr p 2, Tyr p 3, Tyr p 7, Tyr p 8, Tyr p 10 & Tyr p 20) was suitable for Tp-specific diagnosis. Panels of Tp allergens were generated and developed a diagnostic kit able beneficial to identify IgE-mediated Tp hypersensitivity.
螨过敏原是过敏性疾病发生的重要因素。仓储螨（Tp）出现在受污染的食物和家庭环境中。目前用于Tp过敏的诊断工具大多基于粗提物，仍存在缺陷。本研究旨在调查Tp过敏患者的免疫球蛋白E（IgE）反应谱，并开发一种使用重组过敏原的分子诊断方法。将变应原成分表征为交叉反应性或种特异性变应原，其中开发了重组变应原的有效组合，并根据临床诊断的预测准确性进行了分析。克隆并产生了七种重组变应原，以检测Tp过敏的IgE反应性。一项关于螨过敏患病率的调查显示，对屋尘螨（HDM）的IgE反应性致敏率（78.9 - 80.9%）高于对仓储螨Tp的致敏率（35.6%）。老年受试者对Tp的致敏率更高。Tp的主要IgE结合成分是Tyr p 1、Tyr p 2和Tyr p 3。IgE反应性组合D（Tyr p 1、Tyr p 2和Tyr p 3）对Tp过敏的预测准确性很高（100%）。避免交叉反应，IgE反应性组合H +（Tyr p 1、Tyr p 2、Tyr p 3、Tyr p 7、Tyr p 8、Tyr p 10和Tyr p 20）的预测准确性适用于Tp特异性诊断。生成了Tp变应原面板，并开发了一种有助于识别IgE介导的Tp超敏反应的诊断试剂盒。
