Munhbayarlah S, Park J W, Ko S H, Ree H I, Hong C S
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 1998 Apr;39(2):109-15. doi: 10.3349/ymj.1998.39.2.109.
House dust mites are the most common cause of allergic sensitization in respiratory allergic patients in the world. Tyrophagus putrescentiae (TP), which was followed by Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus (DP), has been reported as the third most common house mite in Korea. We previously reported that many respiratory allergic patients had become concomitantly sensitized to DP, DF and TP. The aims of this study were to identify the allergic components of TP and to evaluate the cross-reactivity between TP and DP. The allergenic components of TP and DP extracts were determined with SDS-PAGE and IgE immunoblotting analysis. The cross-reactivity as evaluated by ELISA inhibition and inhibitory immunoblotting experiments. According the SDS-PAGE, the protein components of the two extracts were somewhat different, although a few components displayed identical molecular weights. The 18 kD protein of TP was the most prevalent allergen in the sera of patients sensitized to TP and DP. Both of the maximum inhibition percentages of optic densities of TP-specific IgE in ELISA with TP and DP extract were 100%, respectively and the 50% inhibitory dose (ID50) of TP extract and DP extract were 0.01 micrograms/ml and 0.02 micrograms/ml, respectively. Maximum inhibition of optic densities of DP-specific IgE in ELISA with TP and DP extracts were 29% and 100%, respectively and the ID50 of DP extract was 0.007 micrograms/ml. On inhibitory immunoblotting of DP specific IgE, 5 micrograms/ml of TP extract completely inhibited 16 kD without inhibiting the other allergic component. Whereas, on inhibitory immunoblotting of TP-specific IgE, 5 micrograms/ml of DP extract completely inhibited all the IgE binding components of TP. These results suggested that the major allergen of TP may be the 18 kD component and we also concluded that TP allergens have a strong cross-reactivity with DP extracts, but that DP allergens only have partial cross-reactivity with TP extracts.
屋尘螨是全球呼吸道过敏患者过敏致敏的最常见原因。腐食酪螨（TP）是继粉尘螨（DF）和户尘螨（DP）之后，在韩国被报道的第三大常见屋螨。我们之前报道过，许多呼吸道过敏患者会同时对DP、DF和TP致敏。本研究的目的是鉴定TP的过敏成分，并评估TP与DP之间的交叉反应性。通过SDS-PAGE和IgE免疫印迹分析确定TP和DP提取物的变应原成分。通过ELISA抑制和抑制性免疫印迹实验评估交叉反应性。根据SDS-PAGE结果，两种提取物的蛋白质成分略有不同，尽管有一些成分显示出相同的分子量。TP的18 kD蛋白是对TP和DP致敏患者血清中最普遍的变应原。用TP和DP提取物进行ELISA时，TP特异性IgE光密度的最大抑制百分比均为100%，TP提取物和DP提取物的50%抑制剂量（ID50）分别为0.01微克/毫升和0.02微克/毫升。用TP和DP提取物进行ELISA时，DP特异性IgE光密度的最大抑制率分别为29%和100%，DP提取物的ID50为0.007微克/毫升。在DP特异性IgE的抑制性免疫印迹中，5微克/毫升的TP提取物完全抑制了16 kD蛋白，而没有抑制其他过敏成分。然而，在TP特异性IgE的抑制性免疫印迹中，5微克/毫升的DP提取物完全抑制了TP的所有IgE结合成分。这些结果表明，TP的主要变应原可能是18 kD成分，我们还得出结论，TP变应原与DP提取物有很强的交叉反应性，但DP变应原与TP提取物只有部分交叉反应性。
