School of Food science, International University of Korea, Sangmun-ri, Munsan-eup, Jinju-si, Gyeongnam 660-759, Korea.
Nutr Res Pract. 2010 Feb;4(1):11-5. doi: 10.4162/nrp.2010.4.1.11. Epub 2010 Feb 24.
The purpose of the present study was to investigate the effect of ethanol extracts from red pepper seeds on the antioxidative defense system and oxidative stress in rats fed a high fat . high cholesterol diet. Rats were divided into four experimental groups which were composed of high fat . high cholesterol diet group (HF), high fat . high cholesterol diet with 0.1% ethanol extracts from red pepper seeds supplemented group (HEA), high fat . high cholesterol diet with 0.2% ethanol extracts from red pepper seeds supplemented group (HEB) and high fat.high cholesterol diet with 0.5% ethanol extracts from red pepper seeds supplemented group (HEC). Supplementation of ethanol extracts from red pepper seeds groups (HEA, HEB and HEC) resulted in significantly increased activities of hepatic glutathione peroxidase and catalase. Hepatic superoxide radical contents in microsome and mitochondria were significantly reduced in the groups supplemented with red pepper seeds ethanol extracts. Hepatic hydrogen peroxide content in the mitochondria was reduced in ethanol extracts from red pepper seeds supplemented groups. TBARS values in the liver were reduced in red pepper seeds ethanol extracts supplemented groups. Especially, HEB and HEC groups were significantly decreased compared to the HF group. Hepatic carbonyl values were significantly reduced in mitochondria in these supplemented groups. These results suggest that red pepper seeds ethanol extracts may reduce oxidative damage, by activation of antioxidative defense system in rats fed high fat . high cholesterol diets.
本研究旨在探讨红辣椒籽乙醇提取物对高脂高胆固醇饮食大鼠抗氧化防御系统和氧化应激的影响。将大鼠分为四个实验组，分别为高脂高胆固醇饮食组（HF）、添加 0.1%红辣椒籽乙醇提取物组（HEA）、添加 0.2%红辣椒籽乙醇提取物组（HEB）和添加 0.5%红辣椒籽乙醇提取物组（HEC）。红辣椒籽乙醇提取物组（HEA、HEB 和 HEC）的补充使肝组织谷胱甘肽过氧化物酶和过氧化氢酶的活性显著增加。添加红辣椒籽乙醇提取物可显著降低肝微粒体和线粒体中超氧自由基的含量。添加红辣椒籽乙醇提取物使线粒体中肝组织过氧化氢的含量降低。红辣椒籽乙醇提取物组的肝组织 TBARS 值降低。尤其是 HEB 和 HEC 组与 HF 组相比显著降低。这些补充剂组使线粒体中的肝组织羰基值显著降低。这些结果表明，红辣椒籽乙醇提取物可能通过激活抗氧化防御系统来减轻高脂高胆固醇饮食大鼠的氧化损伤。
