School of Marine Biomedical Sciences, Jeju National University, 1, Ara 1-dong, 102 Jejudaehak-ro, Jeju 690-756, Korea.
Nutr Res Pract. 2011 Oct;5(5):389-95. doi: 10.4162/nrp.2011.5.5.389. Epub 2011 Oct 28.
In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of methanol extract of CPCs prepared by far-infrared radiation (FIR) drying against H(2)O(2)-induced DNA damage was evaluated versus that of freeze-dried CPCs. Methanol extract of FIR-dried CPCs exhibited comparatively good ROS scavenging activity versus the freeze-dried CPCs at the concentration of 100 µg/mL. The extract strongly enhanced the cell viability against H(2)O(2)-induced oxidative damage in Vero cells. Lipid peroxidation inhibitory activity of the extract from FIR-dried CPCs was comparable to that of the extract from freeze-dried CPCs. This sample also exhibited good protective effects against H(2)O(2)-mediated cell apoptosis as demonstrated by decreased apoptotic body formation in the nuclear staining with Hoechst 33342. In the comet assay, the CPC extracts exhibited strong inhibitory effects against H(2)O(2)-mediated DNA damage in a dose-dependent manner. Thus, this study demonstrated that FIR drying effectively preserves CPC as a functionally important natural antioxidant source and the FIR drying can be adapted for drying CPCs and is more economical for massive production than freeze drying.
在本研究中，开发了一种适用于柑橘压榨饼（CPC）的干燥方法，CPC 是柑橘汁厂的副产品。并评估了远红外辐射（FIR）干燥制备的 CPC 甲醇提取物相对于冻干 CPC 对 H₂O₂诱导的 DNA 损伤的保护作用。与冻干 CPC 相比，100μg/ml 浓度下，FIR 干燥的 CPC 甲醇提取物表现出较好的 ROS 清除活性。该提取物能强烈增强 Vero 细胞对 H₂O₂诱导的氧化损伤的细胞活力。FIR 干燥 CPC 提取物的脂质过氧化抑制活性与冻干 CPC 提取物相当。该样品还表现出对 H₂O₂介导的细胞凋亡的良好保护作用，这表现为用 Hoechst 33342 核染色时凋亡小体形成减少。在彗星试验中，CPC 提取物以剂量依赖的方式对 H₂O₂介导的 DNA 损伤表现出强烈的抑制作用。因此，本研究表明 FIR 干燥可有效保持 CPC 作为具有重要功能的天然抗氧化剂来源，并且 FIR 干燥可适用于 CPC 的干燥，并且比冷冻干燥更经济实惠，适用于大规模生产。
