Dipartimento di Chimica, Università della Calabria, cubo 12/C, I-87036 Arcavacata di Rende (CS), Italy.
J Mass Spectrom. 2010 Apr;45(4):358-63. doi: 10.1002/jms.1718.
Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene) is an active component found in several plant species, exhibiting important pharmacological properties. A new and reliable method of assaying this phyto compound in various matrices is presented; the assay is based on (1) the selectivity of liquid chromatography (LC) hyphenated with electrospray ionisation (ESI), (2) the specificity of a two-step mass spectrometric analysis (MS/MS) and (3) the accuracy of the isotope dilution method. The labelled analogue may be conveniently synthesised in a few steps. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and limit of quantitation (LOQ) values achieved in the assay of pterostilbene in two distinct fortified matrices, and is further supported by the observed accuracy values.
紫檀芪（反式-3,5-二甲氧基-4-羟基二苯乙烯）是几种植物中发现的一种活性成分，具有重要的药理学性质。本文提出了一种新的、可靠的测定各种基质中这种植物化合物的方法；该方法基于（1）液相色谱（LC）与电喷雾电离（ESI）的选择性，（2）两步质谱分析（MS/MS）的特异性，以及（3）同位素稀释法的准确性。标记类似物可以方便地通过几步合成。该方法的灵敏度通过在两种不同强化基质中测定紫檀芪时获得的非常低的检测限（LOD）和定量限（LOQ）值得到证实，并通过观察到的准确度值得到进一步支持。
