Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
J Biomed Mater Res A. 2010 Aug;94(2):611-8. doi: 10.1002/jbm.a.32751.
The roughness and cleanness of a titanium surface must be controlled in order to investigate the expression mechanism of hard tissue compatibility on titanium. In this study, osteogenic MC3T3-E1 cells were cultured and differentiation-induced on bulk and sputter-deposited titanium specimens, and the osteogenesis were investigated. For the preparation of bulk specimens, titanium discs were mirror-polished. On the other hand, titanium was sputter-deposited on smooth and clean cover glasses as sputter-deposited specimens. As a result, no significant difference was observed in the cell morphology and attached number. On the other hand, the time showing maximum activity in the alkaline phosphatase and gene expressions, which are related to bone differentiation on the bulk titanium, were superior to those on the sputter-deposited titanium. From the surface observation of the specimens with a scanning electron microscope and a scanning probe microscope, the surface on the sputter-deposited titanium was more uniform and cleaner than that on the bulk titanium. According to X-ray photoelectron spectroscopy, the thickness of surface oxide film on the sputter-deposited titanium was smaller than that on the bulk titanium. In addition, the proportions of TiO and Ti(2)O(3) in the surface oxide film on the sputter-deposited titanium were larger than those on the bulk titanium. These differences might influence the differentiation of osteoblastic cells.
为了研究钛表面的硬组织相容性表达机制，必须控制钛表面的粗糙度和清洁度。在这项研究中，成骨细胞 MC3T3-E1 在块状和溅射沉积钛标本上培养和诱导分化，并对成骨作用进行了研究。为了制备块状标本，钛盘进行了镜面抛光。另一方面，钛被溅射沉积在光滑清洁的载玻片上作为溅射沉积标本。结果表明，细胞形态和附着数量没有显著差异。另一方面，在碱性磷酸酶和与骨分化相关的基因表达方面，最大活性出现的时间在块状钛上优于在溅射沉积钛上。从扫描电子显微镜和扫描探针显微镜观察标本的表面，溅射沉积钛的表面比块状钛更均匀、更清洁。根据 X 射线光电子能谱，溅射沉积钛表面氧化膜的厚度小于块状钛。此外，溅射沉积钛表面氧化膜中 TiO 和 Ti(2)O(3)的比例大于块状钛。这些差异可能会影响成骨细胞的分化。
