Le Guehennec Laurent, Lopez-Heredia Marco-Antonio, Enkel Benedicte, Weiss Pierre, Amouriq Yves, Layrolle Pierre
INSERM U791, Laboratory for Osteo-Articular and Dental Tissue Engineering, Faculty of Dental Surgery, University of Nantes, 1 Place Alexis Ricordeau, 44042 Nantes, France.
Acta Biomater. 2008 May;4(3):535-43. doi: 10.1016/j.actbio.2007.12.002. Epub 2007 Dec 10.
The osseointegration of oral implants is related to the early interactions between osteoblastic cells and titanium surfaces. The behaviour of osteoblastic MC3T3-E1 cells was compared on four different titanium surfaces: mirror-polished (Smooth-Ti), alumina grit-blasted (Alumina-Ti) or biphasic calcium phosphate ceramic grit-blasted (BCP-Ti) and a commercially available implant surface (SLA). Scanning electron microscopy and profilometry showed distinct microtopographies. The BCP-Ti group had higher average surface roughness (Ra=2.5 microm) than the other grit-blasted groups. Hydrophilicity and surfaces energies were determined on the different substrates by dynamic contact angle measurements. The most hydrophilic surface was the Alumina-Ti discs, while SLA was the most hydrophobic. The titanium surfaces were all oxidized as TiO2 and polluted by carbon contaminants, as determined by X-ray photoelectron spectroscopy. Alumina-Ti samples also exhibited aluminium peaks as a result of the blasting. The BCP-Ti discs contained traces of calcium and phosphorus. MC3T3-E1 cells attached, spread and proliferated on the substrates. For both the SLA and BCP-Ti groups, the entire surface was covered with a layer of osteoblastic cells after 2 days. At high magnification, the cells exhibited cytoplasmic extensions and filopodia. Compared with plastic, cell viability was similar with the Smooth-Ti, slightly lower with the Alumina-Ti and superior with the SLA and BCP-Ti groups. Alkaline phosphatase activity increased with the culture time whatever the substrate. This study shows that BCP-blasting produces rough titanium implants without surface contaminants.
口腔种植体的骨结合与成骨细胞和钛表面之间的早期相互作用有关。比较了成骨MC3T3-E1细胞在四种不同钛表面上的行为：镜面抛光（光滑钛）、氧化铝喷砂（氧化铝钛）或双相磷酸钙陶瓷喷砂（BCP钛）以及市售种植体表面（SLA）。扫描电子显微镜和轮廓仪显示出明显的微观形貌。BCP钛组的平均表面粗糙度（Ra = 2.5微米）高于其他喷砂组。通过动态接触角测量确定不同基底上的亲水性和表面能。最亲水的表面是氧化铝钛盘，而SLA是最疏水的。通过X射线光电子能谱测定，钛表面均被氧化为TiO2并被碳污染物污染。由于喷砂，氧化铝钛样品还显示出铝峰。BCP钛盘含有微量的钙和磷。MC3T3-E1细胞在基底上附着、铺展并增殖。对于SLA和BCP钛组，2天后整个表面都覆盖有一层成骨细胞。在高倍放大下，细胞呈现出细胞质延伸和丝状伪足。与塑料相比，光滑钛组的细胞活力相似，氧化铝钛组略低，SLA和BCP钛组则更高。无论基底如何，碱性磷酸酶活性均随培养时间增加。本研究表明，BCP喷砂可产生无表面污染物的粗糙钛种植体。
