Tcheva D, Vladimirov I
Akush Ginekol (Sofiia). 2009;48(2):29-31.
The objective is to report the first healthy live birth in Bulgaria from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM). In vitro maturation protocol was performed in a case with male factor of infertility. Twentyone immature oocytes were retrieved by transvaginal ultrasound guided follicle aspiration on day 10 of a 28-year-old woman and matured in vitro. 28 hours late, 14 of them were in metaphase II. After insemination 13 were fertilizated. Three embryos were transfered at day two. The woman went on to deliver a single healthy live baby at term. We provide proof-of-principle evidence that the novel strategy of infertility treatment by IVM protocol can lead to successful pregnancy and healthy live birth.
目的是报告保加利亚首例通过在自然月经周期中获取未成熟卵母细胞并进行体外成熟（IVM）后实现的健康活产。体外成熟方案应用于一名男性因素不孕的患者。一名28岁女性在月经周期第10天经阴道超声引导下卵泡穿刺获取了21枚未成熟卵母细胞，并进行体外成熟培养。28小时后，其中14枚处于减数分裂中期II。授精后13枚受精。在第2天移植了3枚胚胎。该女性足月分娩了一名健康活婴。我们提供了原理性证据，证明通过IVM方案进行不孕治疗的新策略可导致成功妊娠和健康活产。
