Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, USA.
Anal Chem. 2010 Apr 1;82(7):3094-8. doi: 10.1021/ac100357u.
We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(1) phase synchrony (approximately 84%) and throughput (3 x 10(6) cells/h per microchannel).
我们报告了首次使用超声驻波以高通量且无需试剂的方式实现哺乳动物细胞的细胞周期相位同步。声泳细胞同步(ACS)装置利用微通道内的体积依赖性声辐射力,根据细胞周期依赖性大小波动,从非同步混合物中选择性地纯化所需相位的靶细胞。我们表明,超声分离可实现温和、可扩展且无需标记的同步,具有高 G1 相位同步(约 84%)和高通量(每个微通道 3 x 10(6)个细胞/小时)。