细胞分析中的声流体方法。
Acoustofluidic methods in cell analysis.
作者信息
Xie Yuliang, Bachman Hunter, Huang Tony Jun
机构信息
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
Department of Mechanical Engineering and Material Science, Duke University, Durham, NC 27707, USA.
出版信息
Trends Analyt Chem. 2019 Aug;117:280-290. doi: 10.1016/j.trac.2019.06.034. Epub 2019 Jul 13.
Cellular analysis is a central concept for both biology and medicine. Over the past two decades, acoustofluidic technologies, which marry acoustic waves with microfluidics, have significantly contributed to the development of innovative approaches for cellular analysis. Acoustofluidic technologies enable precise manipulations of cells and the fluids that confine them, and these capabilities have been utilized in many cell analysis applications. In this review article, we examine various applications where acoustofluidic methods have been implemented, including cell imaging, cell mechanotyping, circulating tumor cell phenotyping, sample preparation in clinics, and investigation of cell-cell interactions and cell-environment responses. We also provide our perspectives on the technological advantages, limitations, and potential future directions for this innovative field of methods.
细胞分析是生物学和医学的核心概念。在过去二十年中，将声波与微流体技术相结合的声流体技术，为细胞分析创新方法的发展做出了重大贡献。声流体技术能够精确操控细胞及其周围的流体，这些能力已在许多细胞分析应用中得到利用。在这篇综述文章中，我们研究了已实施声流体方法的各种应用，包括细胞成像、细胞机械分型、循环肿瘤细胞表型分析、临床样本制备以及细胞间相互作用和细胞与环境反应的研究。我们还对这一创新方法领域的技术优势、局限性和潜在的未来发展方向提出了我们的看法。
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