Kusunoki T, Yoshioka Y, Kahda Y, Murata K, Ohta F, Nishida S, Tomura T, Koezuka M
Department of Otolaryngology, Kinki University School of Medicine, Osaka.
Nihon Jibiinkoka Gakkai Kaiho. 1991 Jan;94(1):16-21. doi: 10.3950/jibiinkoka.94.16.
Human thyroid papillary carcinoma cells (surgical materials) were cultured in a monolayer or a collagen gel system. In the monolayer culture, degeneration and necrosis of cancer cells were observed after 10 days of culture. In the collagen gel culture, degenerative necrosis were observed 14 days culture. In histopathological observation monolayer cells proliferated in pavement-like form without forming any colony. On the contrary, collagen gel culture cells formed branching-type colonies and spheroid-type colonies. Those results suggested that papillary carcinoma cells can be cultured more like in vivo in collagen gel culture than monolayer culture.
人甲状腺乳头状癌细胞（手术材料）在单层培养或胶原凝胶体系中培养。在单层培养中，培养10天后观察到癌细胞变性和坏死。在胶原凝胶培养中，培养14天后观察到变性坏死。组织病理学观察显示，单层细胞呈铺路石样增殖，未形成任何集落。相反，胶原凝胶培养细胞形成分支型集落和球样集落。这些结果表明，与单层培养相比，胶原凝胶培养中乳头状癌细胞的培养更接近体内情况。
