CHUQ-CHUL Research Center, Ontogeny and Reproduction, Quebec, QC, Canada.
Acta Obstet Gynecol Scand. 2010 Mar;89(3):360-6. doi: 10.3109/00016340903576020.
Alteration of cytokines level and oxidative stress are both associated with preeclampsia (PE). We have investigated if IL-6 and IL-18 levels were related to coenzyme Q(10) (CoQ(10)), an antioxidant and a marker of oxidative stress in the plasma from normotensive and preeclamptic pregnancies.
IL-6 and IL-18 levels were determined by enzyme-linked immunosorbent assays in plasmas from preeclamptic (n = 29) and normotensive pregnancies (n = 30). The concentrations of CoQ(10) in the different redox forms were measured in plasma using liquid chromatography coupled to electrochemical detection. Data were analyzed using the Wilcoxon Rank-Sum test and correlations were obtained by the Spearman's Rho test.
IL-6 concentrations were 2.8-fold higher in preeclamptic plasmas than in controls (p = 0.0006), and IL-18 concentrations were found significantly lower in preeclamptic samples than in controls (p = 0.007). No correlation was found between IL-18 or IL-6 and antioxidant vitamin CoQ(10) in plasmas from normotensive pregnant women. However, in PE, IL-18 level was positively correlated with the reduced form of CoQ(10) (r = 0.3680, p = 0.0495).
This is the first demonstration that IL-18 is potentially linked to oxidative stress in PE, since its level correlates with the concentration of the powerful antioxidant CoQ(10). These results also associate the immune system with the oxidant/antioxidant imbalance observed in PE.
细胞因子水平的改变和氧化应激都与先兆子痫（PE）有关。我们研究了白细胞介素 6（IL-6）和白细胞介素 18（IL-18）水平是否与辅酶 Q10（CoQ10）有关，CoQ10 是一种抗氧化剂，也是正常妊娠和先兆子痫孕妇血浆中氧化应激的标志物。
采用酶联免疫吸附试验测定 29 例先兆子痫孕妇和 30 例正常妊娠孕妇血浆中的 IL-6 和 IL-18 水平。采用液相色谱-电化学检测法测定不同氧化还原形式的 CoQ10 浓度。采用 Wilcoxon 秩和检验分析数据，采用 Spearman's Rho 检验进行相关性分析。
先兆子痫孕妇血浆中 IL-6 浓度比对照组高 2.8 倍（p = 0.0006），IL-18 浓度明显低于对照组（p = 0.007）。在正常妊娠孕妇的血浆中，IL-18 或 IL-6 与抗氧化维生素 CoQ10 之间均无相关性。然而，在 PE 中，IL-18 水平与 CoQ10 的还原形式呈正相关（r = 0.3680，p = 0.0495）。
这是首次证明 IL-18 与 PE 中的氧化应激有关，因为其水平与强大的抗氧化剂 CoQ10 的浓度相关。这些结果还将免疫系统与 PE 中观察到的氧化剂/抗氧化剂失衡联系起来。
