Fadhil Sabreen Hadi, Saheb Entsar Jabbar
Department of Biology, College of Science, Baghdad University, Baghdad, Iraq.
Iran J Parasitol. 2024 Oct-Dec;19(4):408-417. doi: 10.18502/ijpa.v19i4.17161.
We aimed to shed light on the risks of elevated levels of IL-18 in aborted women with toxoplasmosis by evaluating the risk or protective function of alleles or genotypes for single nucleotide polymorphism (SNP) of IL-18 (rs 1946519), which might be related to the susceptibility to toxoplasmosis.
IL-18 levels in patient and control blood samples were determined using ELISA, and the SNP IL-18 (ra 1946519) was subjected to the high-resolution method.
Compared to healthy pregnant women (HP), the IL-18 serum levels of recurrent abortion with toxoplasmosis (RAWT), recurrent abortion without toxoplasmosis (RAWOT), and healthy non-pregnant (HNP) women decreased with significant differences. Additionally, a strong association between patients and controls was found in the SNP IL-18 data. RAWT and RAWOT with the genotypes AA and AC had significantly lower IL-18 serum levels than women HP, according to the distribution of IL-18 serum levels by SNP.
The serum level of IL-18 varied by genotype in patients with substantial differences compared to controls, while the SNP of IL-18 has been linked as a risk factor in toxoplasmosis-infected recurrent abortion women.
我们旨在通过评估白细胞介素-18（IL-18）单核苷酸多态性（SNP，rs 1946519）的等位基因或基因型的风险或保护功能，来阐明弓形虫病流产妇女中IL-18水平升高的风险，这可能与弓形虫病易感性有关。
使用酶联免疫吸附测定法（ELISA）测定患者和对照血样中的IL-18水平，并采用高分辨率方法检测SNP IL-18（rs 1946519）。
与健康孕妇（HP）相比，弓形虫病复发性流产（RAWT）、无弓形虫病复发性流产（RAWOT）和健康非孕妇（HNP）妇女的IL-18血清水平降低，差异有统计学意义。此外，在SNP IL-18数据中发现患者与对照之间存在强关联。根据SNP的IL-18血清水平分布，基因型为AA和AC的RAWT和RAWOT妇女的IL-18血清水平显著低于HP妇女。
与对照组相比，患者中IL-18的血清水平因基因型而异，差异显著，而IL-18的SNP已被认为是弓形虫病感染的复发性流产妇女的一个风险因素。
