Zahmatkesh Maryam, Kadkhodaee Mehri, Mahdavi-Mazdeh Mitra, Ghaznavi Rana, Hemati Mohamad, Seifi Behjat, Golab Fereshteh, Hasani Keyvan, Lessan-Pezeshki Mahbob, Einollahi Behzad
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Exp Clin Transplant. 2010 Mar;8(1):38-44.
Despite the demonstration of oxidative stress in patients with end-stage renal disease, the oxidative status during and after a renal transplant are not completely understood. Hepatocyte growth factor is reported to act as an endogenous factor against oxidative stress. The aim of this study was to evaluate the pattern of changes in plasma oxidative status and hepatocyte growth factor levels in living-donor renal transplant recipients during the early phase after transplant.
Nineteen patients who underwent a renal transplant were included. All were on cyclosporine-based immunosuppression. Plasma levels of malondialdehyde, ferric reducing activity, hepatocyte growth factor, vitamin E, erythrocyte glutathione, and superoxide dismutase activities were determined before, and on the second, seventh, and 12th days after the transplant.
High malondialdehyde concentration and low superoxide dismutase activity were seen before and 48 hours after transplant compared with healthy subjects. Significant reductions in plasma ferric reducing activity, malondialdehyde, and hepatocyte growth factor were seen on the seventh and twelfth days after transplant, compared with the before-transplant data. Direct correlations were found between hepatocyte growth factor levels and ferric reducing activity of plasma as well as hepatocyte growth factor and creatinine or uric acid.
Renal transplant recipients display persistent oxidative stress during the early phase of transplant. The pattern of oxidative changes should be considered for appropriate time, dosage, type, and the duration of antioxidant therapy in these patients.
尽管已证实终末期肾病患者存在氧化应激，但肾移植期间及术后的氧化状态尚未完全明确。据报道，肝细胞生长因子可作为一种内源性因子抵抗氧化应激。本研究旨在评估活体供肾移植受者移植后早期血浆氧化状态及肝细胞生长因子水平的变化模式。
纳入19例接受肾移植的患者。所有患者均接受以环孢素为基础的免疫抑制治疗。在移植前、移植后第2天、第7天和第12天测定血浆丙二醛、铁还原活性、肝细胞生长因子、维生素E、红细胞谷胱甘肽和超氧化物歧化酶活性。
与健康受试者相比，移植前及移植后48小时可见高丙二醛浓度和低超氧化物歧化酶活性。与移植前数据相比，移植后第7天和第12天血浆铁还原活性、丙二醛和肝细胞生长因子显著降低。发现肝细胞生长因子水平与血浆铁还原活性以及肝细胞生长因子与肌酐或尿酸之间存在直接相关性。
肾移植受者在移植早期表现出持续的氧化应激。在这些患者中，应考虑氧化变化模式以确定抗氧化治疗的合适时间、剂量、类型和持续时间。
