Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
Biomaterials. 2010 May;31(15):4322-9. doi: 10.1016/j.biomaterials.2010.02.008. Epub 2010 Mar 2.
To form tissues with uniform cell distribution and extracellular matrix arrangement is of great relevance to obtain the desirable function and maintain structural integrity. Scaffold configuration is believed to play a critical role in regulating cell spatial distribution and consequently tissue formation. In this study, three types of poly(ethyleneglycol-terephthalate)-poly (butylenes terephthalate) (PEGT/PBT) scaffolds [compression molded scaffold (CM), compression molded scaffold after chloroform/isopropanol reticulation (CMR), 3D rapid prototyped fibrous scaffold (RP)] with various configurations were used to support the tissue formation of adipose stromal cells for up to 21 days. Characterization of the scaffolds with muCT revealed that RP scaffolds were composed of repeating structural units with well controlled interconnected pores, in contrast to the irregular pore morphology in CM or CMR. Cell seeding efficacy onto various scaffolds was comparable (from 67 +/- 4% to 82 +/- 3%), while only RP scaffold led to even cell attachment onto the inner fibers of the scaffolds. Continuous cell proliferation and deposition of new collagen and glycosaminoglycans (GAG) were measured for all three scaffolds, while with a significant amount measured in RP at 21 days. By 21 days, complete uniform tissue formation was only achieved in RP scaffolds under a dynamic cell culture in spinner flasks. The present study successfully demonstrates the feasibility of controlling uniform tissue formation at a microscale by manipulating the structural configuration of the scaffold.
形成具有均匀细胞分布和细胞外基质排列的组织对于获得理想的功能和维持结构完整性非常重要。支架结构被认为在调节细胞空间分布和组织形成方面起着关键作用。在这项研究中，使用了三种不同结构的聚（乙二醇-对苯二甲酸酯）-聚（对苯二甲酸丁二醇酯）（PEGT/PBT）支架[压缩成型支架（CM）、氯仿/异丙醇交联后的压缩成型支架（CMR）、3D 快速原型纤维支架（RP）]来支持脂肪基质细胞的组织形成，时间长达 21 天。使用 muCT 对支架进行了表征，结果表明，RP 支架由具有良好控制的互连孔的重复结构单元组成，而 CM 或 CMR 则具有不规则的孔形态。细胞在各种支架上的接种效率相当（从 67%±4%到 82%±3%），而只有 RP 支架才能使细胞均匀地附着在支架的内纤维上。对所有三种支架都测量了连续的细胞增殖和新胶原蛋白和糖胺聚糖（GAG）的沉积，而在 RP 支架中在 21 天时测量到了大量的沉积。在旋转瓶中的动态细胞培养下，只有在 RP 支架中，21 天后才能完全均匀地形成组织。本研究成功地证明了通过操纵支架的结构配置来控制微尺度均匀组织形成的可行性。
