1 Department of Basic Medical Sciences, Ghent University , Ghent, Belgium .
Tissue Eng Part A. 2014 Jan;20(1-2):434-44. doi: 10.1089/ten.TEA.2013.0179. Epub 2013 Oct 17.
Scaffold architecture and composition are crucial parameters determining the initial cell spatial distribution and consequently bone tissue formation. Three-dimensional poly-ε-caprolactone (PCL) scaffolds with a 0/90° lay-down pattern were plotted and subjected to (1) an oxygen plasma (PCL O) or (2) a postargon plasma modification with gelatin and fibronectin (PCL Fn). These scaffolds with an open pore structure were compared with more compact scaffolds fabricated by conventional processing techniques: oxidized polylactic acid (LA O) and collagen (COL) scaffolds. Human adipose tissue-derived stem cell/scaffold interactions were studied. The study revealed that the biomimetic surface modification of plotted scaffolds did not increase the seeding efficiency. The proliferation and colonization was superior for PCL Fn in comparison with PCL O. The plotted PCL Fn was completely colonized throughout the scaffold, whereas conventional scaffolds only at the edge. Protein-based scaffolds (PCL Fn and COL) enhanced the differentiation, although plotted scaffolds showed a delay in their differentiation compared with compact scaffolds. In conclusion, protein modification of plotted PCL scaffolds enhances uniform tissue formation, but shows a delayed differentiation in comparison with compact scaffolds. The present study demonstrates that biomimetic PCL scaffolds could serve as a guiding template to obtain a uniform bone tissue formation in vivo.
支架结构和组成是决定细胞初始空间分布进而影响骨组织形成的关键参数。本文设计了一种 0/90°铺层图案的聚己内酯（PCL）支架，并对其进行了（1）氧等离子体（PCL O）或（2）与明胶和纤维连接蛋白（PCL Fn）的后氩等离子体修饰。将这种具有开放孔隙结构的支架与通过传统加工技术制备的更致密的支架（氧化聚乳酸（LA O）和胶原（COL）支架）进行了比较。研究了人脂肪组织源性干细胞/支架的相互作用。研究表明，仿生表面修饰并未提高支架的接种效率。与 PCL O 相比，PCL Fn 更有利于细胞的增殖和定植。PCL Fn 完全填充了支架的整个孔隙，而传统支架仅在边缘处被细胞填充。基于蛋白质的支架（PCL Fn 和 COL）增强了分化，尽管与致密支架相比，图案化支架的分化有所延迟。总之，与致密支架相比，PCL 支架的蛋白质修饰增强了组织的均匀形成，但分化延迟。本研究表明，仿生 PCL 支架可用作模板，以获得体内均匀的骨组织形成。
