Central Veterinary Institute of Wageningen UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
J Antimicrob Chemother. 2010 May;65(5):872-5. doi: 10.1093/jac/dkq049. Epub 2010 Mar 3.
To characterize plasmids and resistance genes of multidrug-resistant (MDR) Salmonella Senftenberg and Salmonella Concord isolated from patients in the Netherlands.
The resistance genes of four MDR Salmonella isolates (three Salmonella Concord and one Salmonella Senftenberg) were identified by miniaturized microarray, PCR and sequencing. Plasmids were characterized by S1 nuclease-PFGE and PCR-based replicon typing (PBRT). Linkage between plasmids and genes was determined by conjugation experiments and microarray analysis. The genetic relationship between the three Salmonella Concord isolates was determined by XbaI-PFGE.
A large variety of resistance genes was detected, including qnrB2 and the beta-lactamase genes bla(TEM-1) and bla(SHV-12) in all isolates; moreover all Salmonella Concord isolates also harboured bla(CTX-M-15). Salmonella Senftenberg harboured a large IncHI2 plasmid. The three Salmonella Concord isolates harboured two large plasmids typed as IncHI2 and IncA/C.
We detected the first plasmid-mediated MDR Salmonella isolates in the Netherlands harbouring both qnr and extended-spectrum beta-lactamase (ESBL) genes. In Salmonella Senftenberg one large plasmid (IncHI2) and in Salmonella Concord two large plasmids (IncHI2 and IncA/C) were responsible for the multidrug resistance.
对来自荷兰患者的多药耐药（MDR）肠炎沙门氏菌和肠炎沙门氏菌分离株的质粒和耐药基因进行特征分析。
通过微型芯片、PCR 和测序鉴定了 4 株 MDR 肠炎沙门氏菌分离株（3 株肠炎沙门氏菌和 1 株肠炎沙门氏菌）的耐药基因。通过 S1 核酸酶-PFGE 和基于 PCR 的复制子分型（PBRT）对质粒进行了特征分析。通过接合实验和微阵列分析确定质粒和基因之间的联系。通过 XbaI-PFGE 确定了 3 株肠炎沙门氏菌分离株之间的遗传关系。
检测到多种耐药基因，包括所有分离株中的 qnrB2 和 bla(TEM-1) 和 bla(SHV-12)β-内酰胺酶基因；此外，所有肠炎沙门氏菌分离株还携带 bla(CTX-M-15)。肠炎沙门氏菌携带一个大型 IncHI2 质粒。3 株肠炎沙门氏菌分离株携带两种类型为 IncHI2 和 IncA/C 的大型质粒。
我们在荷兰检测到首例携带 qnr 和超广谱β-内酰胺酶（ESBL）基因的质粒介导的多药耐药肠炎沙门氏菌分离株。在肠炎沙门氏菌中，一个大质粒（IncHI2）和在肠炎沙门氏菌中，两个大质粒（IncHI2 和 IncA/C）负责多药耐药。
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