Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, People's Republic of China.
Pest Manag Sci. 2010 Jun;66(6):669-75. doi: 10.1002/ps.1928.
Twenty-three isolates of Metarhizium anisopliae (Metschnikof) Sorokin and M. acridum (Driver & Milner) JF Bischoff, Rehner & Humber from non-aphid host insects around the globe were evaluated for their aphid biocontrol potential, which is not well known.
The apterous adults of green peach aphid Myzus persicae (Sulzer) were exposed to the fungal sprays of 11.5, 99 and 1179 conidia mm(-2) and blank control in three leaf-dish bioassays. All the tested isolates except one were proven to be infective to the aphid species at 21 +/- 1 degrees C and 14:10 h light:dark photoperiod, causing corrected mortalities of 10.1-95.3% at the high spore concentration. The data from ten isolates causing > 50% mortality at the high concentration were found to fit a time-concentration-mortality model well, yielding parameters for the estimates of their LC(50) and LT(50) that vary with post-spray time and spore concentration respectively. Four isolates of M. anisopliae (ARSEF 759, 4132, 2080 and 576) had LC(50) values of 44-80 conidia mm(-2) on day 8 and LT(50) values of 4.9-6.8 days at 100 conidia mm(-2), with 91-98% of the killed aphids being well mycotised after death.
The Metarhizium infectivity to M. persicae differs greatly among the tested isolates. The four mentioned isolates with desired virulence and sporulation potential are excellent candidates for microbial control of aphids.
从全球非蚜虫宿主昆虫中评估了 23 株绿僵菌（Metschnikof）Sorokin 和 M. acridum（Driver＆Milner）JF Bischoff、Rehner＆Humber 的蚜虫生物防治潜力，而这方面的了解并不多。
在三个叶碟生物测定中，用 11.5、99 和 1179 个分生孢子/mm²和空白对照喷雾处理无翅桃蚜 Myzus persicae（Sulzer）的成虫。在 21±1°C 和 14:10 h 光：暗光周期下，除一个分离株外，所有测试分离株均被证明对蚜虫具有感染力，在高孢子浓度下引起校正死亡率为 10.1-95.3%。在高浓度下引起>50%死亡率的十个分离株的数据拟合时间-浓度-死亡率模型非常好，得出了估计其 LC（50）和 LT（50）的参数，分别随喷雾后时间和孢子浓度而变化。四株绿僵菌（ARSEF 759、4132、2080 和 576）的 LC（50）值分别为 44-80 个分生孢子/mm²，在第 8 天，在 100 个分生孢子/mm²时 LT（50）值为 4.9-6.8 天，死亡后 91-98%的蚜虫被很好地真菌化。
测试的分离株中，绿僵菌对桃蚜的感染力有很大差异。这四个具有所需毒力和产孢潜力的分离株是微生物防治蚜虫的优秀候选物。
