Department of Chemical Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China.
J Biomed Nanotechnol. 2009 Oct;5(5):495-504. doi: 10.1166/jbn.2009.1074.
Pyrene excimer nucleic acid probes are novel molecular probes that integrate the unique optical properties of pyrene excimer with excellent recognition capability of nucleic acids. Pyrene has the property of forming excimer that has a large Stokes shift and long fluorescence lifetime. Nucleic acid probes, like aptamers and molecular beacons, can directly bind to target molecules with a high selectivity and affinity. Possessing these outstanding characteristics of pyrene excimer and nucleic acid probes, pyrene excimer nucleic acid probes can be effectively used in detection of biomolecules, study of protein-DNA interactions, and investigation of enzymatic processes in complex biological systems. In this review, we will discuss the optical properties of pyrene excimer and summarize recent progresses on pyrene excimer signaling nucleic acid probes for the signaling of biomolecules and their interactions in complex biological samples.
芘荧光素二聚体核酸探针是一种新型的分子探针，它将芘荧光素二聚体的独特光学性质与核酸的优异识别能力相结合。芘具有形成激基缔合物的性质，具有较大的斯托克斯位移和长荧光寿命。核酸探针，如适体和分子信标，可以与靶分子直接结合，具有高选择性和亲和力。芘荧光素二聚体和核酸探针具有这些优异的特性，芘荧光素二聚体核酸探针可以有效地用于生物分子的检测、蛋白质-DNA 相互作用的研究以及复杂生物系统中酶过程的研究。在这篇综述中，我们将讨论芘荧光素二聚体的光学性质，并总结近年来关于芘荧光素二聚体信号核酸探针在复杂生物样品中生物分子及其相互作用信号方面的研究进展。
