School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, China.
Biomacromolecules. 2010 Apr 12;11(4):889-95. doi: 10.1021/bm901241k.
Over the past 10 years, polyglycerols and their structurally related analogs have received considerable attention in the biomedical field. Poly(glycidyl methacrylate) (PGMA) is a versatile polymer because its pendant epoxide groups can be opened with different functional groups to generate poly(glycerol methacrylate)s (PGOHMA) derivatives. In this work, linear and star-shape PGMAs were synthesized by atom transfer radical polymerization and then functionalized with four different amines by ring-opening addition. This resulted in the formation of polyglycerol-like polymers having both hydroxyl and amine moieties and different water-solubility. The water-insoluble polymers could form pH-sensitive nanoassemblies, while the soluble derivatives efficiently complexed a short strand polynucleotide. The aminated polyglycerol interacted more avidly with the oligonucleotide than the control poly(ethyleneimine), and high transfection efficacy could be obtained with the linear derivative. Such polymers could find practical applications for the delivery of drugs and nucleic acids.
在过去的 10 年中，聚甘油及其结构相关的类似物在生物医学领域受到了相当大的关注。聚（甲基丙烯酸缩水甘油酯）（PGMA）是一种多功能聚合物，因为其侧链环氧基团可以与不同的官能团开环，生成聚（甘油甲基丙烯酸酯）（PGOHMA）衍生物。在这项工作中，通过原子转移自由基聚合合成了线性和星形 PGMA，然后通过开环加成与四种不同的胺官能化。这导致形成了具有羟基和胺部分以及不同水溶性的类似甘油的聚合物。不溶于水的聚合物可以形成 pH 敏感的纳米组装体，而可溶性衍生物则可以有效地与短链多核苷酸复合。与对照聚（亚乙基亚胺）相比，氨基化聚甘油与寡核苷酸的相互作用更强烈，并且可以通过线性衍生物获得高转染效率。这些聚合物在药物和核酸的递送上可能有实际应用。
