Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, UK.
Anal Chem. 2010 Apr 1;82(7):3073-6. doi: 10.1021/ac902932c.
We present a rapid method for protein tertiary structure analysis which avoids the need for techniques such as circular dichroism and differential scanning calorimetry. Small changes to a protein's noncovalent "soft" structure are detected by exploiting differences in thermal stability and fluorescent reporter binding. It can detect subtle stability differences using micrograms of protein in 2 microL volumes within minutes.
我们提出了一种快速的蛋白质三级结构分析方法，该方法避免了使用圆二色性和差示扫描量热法等技术。通过利用热稳定性和荧光报告分子结合的差异来检测蛋白质非共价“软”结构的微小变化。它可以在数分钟内使用 2 微升体积中的微克级蛋白质检测到细微的稳定性差异。
