Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506-2201, USA.
J Food Prot. 2010 Mar;73(3):568-73. doi: 10.4315/0362-028x-73.3.568.
Laboratory experiments were designed to determine the survival of Enterococcus faecalis OG1RF:pCF10 in poultry and cattle feed and its acquisition and transmission by adults of the red flour beetle, Tribolium castaneum (Herbst), to sterile feed. Adult T. castaneum beetles were introduced into poultry and cattle feed inoculated with E. faecalis OG1RF:pCF10 and incubated at 28 degrees C with 65% relative humidity for 7 days in a growth chamber. E. faecalis survived in both poultry and cattle feed during the 7-day test period. There was a logarithmic decrease in E. faecalis concentration in poultry and cattle feed and in and on the insects. E. faecalis persisted on the surface and within T. castaneum adults for 7 days when adults were released on E. faecalis-inoculated poultry feed and for only 5 days on E. faecalis-inoculated cattle feed. The concentration of E. faecalis decreased more slowly on poultry feed than on cattle feed, and this may explain why adult T. castaneum insects were more successful in acquiring and transferring E. faecalis from inoculated poultry feed to sterile poultry feed during the 7-day test period. However, T. castaneum adults reared on inoculated cattle feed were unable to contaminate sterile cattle feed on day 7. To our knowledge, this is the first report documenting T. castaneum to successfully acquire antibiotic-resistant enterococci from animal feed and transfer them to sterile feed. Management of T. castaneum through effective integrated pest management program is therefore important to prevent the spread of antibiotic-resistant and virulent enterococci in animal feed and feed manufacturing environments.
实验室实验旨在确定屎肠球菌 OG1RF:pCF10 在禽畜饲料中的存活情况，以及其在无菌饲料中被赤拟谷盗成虫（Tribolium castaneum（ Herbst））摄取和传播的情况。将成年赤拟谷盗引入接种了屎肠球菌 OG1RF:pCF10 的禽畜饲料中，并在生长室中于 28°C 和 65%相对湿度下孵育 7 天。在 7 天的测试期内，屎肠球菌在禽畜饲料中均能存活。在禽畜饲料中和昆虫体内和体表，屎肠球菌的浓度呈对数下降。当成年赤拟谷盗被释放到接种了屎肠球菌的禽畜饲料上时，其在表面和体内可存活 7 天，但在接种了屎肠球菌的牛饲料上仅存活 5 天。与牛饲料相比，禽饲料上屎肠球菌的浓度下降较慢，这可能解释了为什么在 7 天的测试期内，成年赤拟谷盗昆虫更成功地从接种了禽饲料的动物饲料中摄取并转移屎肠球菌到无菌禽饲料中。然而，在接种了牛饲料中饲养的赤拟谷盗成虫在第 7 天无法污染无菌牛饲料。据我们所知，这是首次报道赤拟谷盗成功从动物饲料中获取抗生素抗性肠球菌并将其转移到无菌饲料中的情况。因此，通过有效的综合虫害管理计划来管理赤拟谷盗对于防止抗生素抗性和毒力肠球菌在动物饲料和饲料制造环境中的传播非常重要。
