College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Int Immunopharmacol. 2010 May;10(5):611-8. doi: 10.1016/j.intimp.2010.02.013. Epub 2010 Mar 2.
Oligonucleotides containing CpG motifs (CpG ODN) are known to be potent stimulators of the innate immune system in vitro and in vivo. We therefore investigated if intranasal (IN)-mucosal or intramuscular (IM)-systemic administration of CpG ODN could enhance innate immunity in the intestinal mucosa and peripheral blood mononuclear cells (PBMCs) in piglets. Repeated IN or IM administration of CpG ODN significantly increased local/systemic mRNA expression of the CC chemokines macrophage inflammatory protein 1beta (MIP-1beta) and monocyte chemoattractant protein-1 (MCP-1) and CXC chemokine gamma interferon-inducible protein 10 (IP-10) and percentages of macrophages and cDCs in the intestine (jejunum, caecum and colon) and PBMCs by different kinetics. IN delivery of CpG ODN induced much stronger chemokine responses than IM delivery at intestinal mucosas, whereas IN delivery of CpG ODN induced some weaker chemokine responses than IM delivery in PBMCs. These findings suggest that IN administration of 100mug/kg-CpG ODN without antigen codelivery may represent a valuable strategy for induction of innate immunity against infection.
含有 CpG 基序的寡核苷酸（CpG ODN）已知在体外和体内是先天免疫系统的有效刺激物。因此，我们研究了鼻内（IN）黏膜或肌肉内（IM）系统给予 CpG ODN 是否可以增强仔猪肠道黏膜和外周血单核细胞（PBMC）中的先天免疫。CpG ODN 的重复 IN 或 IM 给药显著增加了局部/全身 CC 趋化因子巨噬细胞炎性蛋白 1β（MIP-1β）和单核细胞趋化蛋白-1（MCP-1）和 CXC 趋化因子γ干扰素诱导蛋白 10（IP-10）的 mRNA 表达以及肠道（空肠、盲肠和结肠）和 PBMC 中巨噬细胞和 cDCs 的百分比。CpG ODN 的 IN 给药在肠道黏膜处诱导的趋化因子反应比 IM 给药强得多，而 IN 给药在 PBMC 中诱导的趋化因子反应比 IM 给药弱一些。这些发现表明，在没有抗原共给药的情况下，100μg/kg-CpG ODN 的 IN 给药可能是诱导针对感染的先天免疫的一种有价值的策略。
