Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, Rome, Italy.
Water Res. 2010 May;44(9):2745-52. doi: 10.1016/j.watres.2010.02.008. Epub 2010 Feb 11.
This study investigates the anaerobic treatment of an industrial wastewater from a Fischer-Tropsch (FT) process in a continuous-flow packed-bed biofilm reactor operated under mesophilic conditions (35 degrees C). The considered synthetic wastewater has an overall chemical oxygen demand (COD) concentration of around 28g/L, mainly due to alcohols. A gradual increase of the organic load rate (OLR), from 3.4gCOD/L/d up to 20gCOD/L/d, was adopted in order to overcome potential inhibitory effects due to long-chain alcohols (>C6). At the highest applied OLR (i.e., 20gCOD/L/d) and a hydraulic retention time of 1.4d, the COD removal was 96% with nearly complete conversion of the removed COD into methane. By considering a potential of 200tCOD/d to be treated, this would correspond to a net production of electric energy of about 8x10(7)kWh/year. During stable reactor operation, a COD balance and batch tests showed that about 80% of the converted COD was directly metabolized through H(2)(-) and acetate-releasing reactions, which proceeded in close syntrophic cooperation with hydrogenotrophic and acetoclastic methanogenesis (contributing to about 33% and 54% of overall methane production, respectively). Finally, energetic considerations indicated that propionic acid oxidation was the metabolic conversion step most dependent on the syntrophic partnership of hydrogenotrophic methanogens and accordingly the most susceptible to variations of the applied OLR or toxicity effects.
本研究考察了在中温条件（35°C）下连续流填充床生物膜反应器中，用 Fischer-Tropsch（FT）工艺处理工业废水的厌氧处理。所考虑的合成废水的总化学需氧量（COD）浓度约为 28g/L，主要是由于醇类。采用逐渐增加有机负荷率（OLR）的方法，从 3.4gCOD/L/d 增加到 20gCOD/L/d，以克服由于长链醇（>C6）引起的潜在抑制作用。在应用的最高 OLR（即 20gCOD/L/d）和水力停留时间为 1.4d 的情况下，COD 去除率为 96%，去除的 COD 几乎完全转化为甲烷。考虑到有 200tCOD/d 的潜在处理量，这将对应于每年约 8x10(7)kWh 的净电能生产。在稳定的反应器运行期间，COD 平衡和分批试验表明，约 80%的转化 COD 直接通过 H(2)(-)和乙酸释放反应进行代谢，这些反应与氢营养型和乙酸营养型甲烷生成密切协同（分别对总甲烷生成的约 33%和 54%作出贡献）。最后，能量考虑表明，丙酸氧化是最依赖于氢营养型产甲烷菌的协同作用的代谢转化步骤，因此最容易受到应用的 OLR 或毒性效应的变化的影响。
