Department of Materials Science and Engineering, The University of Tennessee, Knoxville, TN 37996, USA.
Biomaterials. 2010 Jun;31(16):4457-66. doi: 10.1016/j.biomaterials.2010.02.020. Epub 2010 Mar 4.
We present a method to modify poly(propylene fumarate) (PPF), an injectable biomaterial for bone-tissue-engineering applications, by photo-crosslinking it with methoxy poly(ethylene glycol) monoacrylate (mPEGA) at various mPEGA compositions of 0-30%. The bulk properties such as thermal and rheological properties of uncrosslinked mPEGA/PPF blends and the mechanical properties of photo-crosslinked mPEGA/PPF blends were also investigated and correlated with surface characteristics to elaborate on the modulation of mouse MC3T3 cell adhesion, spreading, proliferation and differentiation through controlled physicochemical properties. Unlike PPF crosslinked with PEG dimethacrylate, mPEGA chains tethered on the surface of crosslinked PPF did not influence the swelling ratio in water while increased surface hydrophilicity greatly. Meanwhile, surface frictional coefficient and the capability of adsorbing proteins from cell culture medium decreased continuously with increasing the mPEGA composition in mPEGA/PPF networks. Demonstrating cell repulsive effect at the mPEGA compositions higher than 7%, the modified surfaces improved MC3T3 cell attachment, proliferation and differentiation, which reached maxima at the mPEGA composition of 5-7%. Besides revealing that mPEGA pendant chains could enhance cell responses by increasing hydrophilicity when their fraction on the hydrophobic surface was small, the present study also offered a new method of improving the wettability and performance of the scaffolds made from PPF for bone repair.
我们提出了一种方法来修饰聚（富马酸丙二醇酯）（PPF），一种用于骨组织工程应用的可注射生物材料，通过将其与甲氧基聚（乙二醇）单丙烯酸酯（mPEGA）在 0-30%的各种 mPEGA 组成下进行光交联。还研究了未交联的 mPEGA/PPF 共混物的热和流变性能以及光交联的 mPEGA/PPF 共混物的机械性能，并与表面特性相关联，以详细阐述通过控制物理化学性质来调节小鼠 MC3T3 细胞的黏附、铺展、增殖和分化。与聚乙二醇二甲基丙烯酸酯交联的 PPF 不同，接枝在交联 PPF 表面上的 mPEGA 链不会影响在水中的溶胀比，而大大增加了表面亲水性。同时，表面摩擦系数和从细胞培养液中吸附蛋白质的能力随着 mPEGA/PPF 网络中 mPEGA 组成的增加而连续降低。在 mPEGA 组成高于 7%时表现出对细胞的排斥作用，修饰后的表面改善了 MC3T3 细胞的附着、增殖和分化，在 mPEGA 组成 5-7%时达到最大值。除了表明 mPEGA 侧链可以通过增加疏水性表面上的亲水性来增强细胞反应，当它们在疏水性表面上的分数较小时，本研究还提供了一种改善由 PPF 制成的支架的润湿性和性能的新方法，用于骨修复。
