Department of Materials Science and Engineering, The University of Tennessee, Knoxville, Tennessee 37996, United States.
Biomacromolecules. 2012 Feb 13;13(2):358-68. doi: 10.1021/bm201372u. Epub 2012 Jan 18.
We present a method of tuning surface chemistry and nerve cell behavior by photo-cross-linking methoxy poly(ethylene glycol) monoacrylate (mPEGA) with hydrophobic, semicrystalline poly(ε-caprolactone) diacrylate (PCLDA) at various weight compositions of mPEGA (ø(m)) from 2 to 30%. Improved surface wettability is achieved with corresponding decreases in friction, water contact angle, and capability of adsorbing proteins from cell culture media because of repulsive PEG chains tethered in the network. The responses of rat Schwann cell precursor line (SpL201), rat pheochromocytoma (PC12), and E14 mouse neural progenitor cells (NPCs) to the modified surfaces are evaluated. Nonmonotonic or parabolic dependence of cell attachment, spreading, proliferation, and differentiation on ø(m) is identified for these cell types with maximal values at ø(m) of 5-7%. In addition, NPCs demonstrate enhanced neuronal differentiated lineages on the mPEGA/PCLDA network at ø(m) of 5% with intermediate wettability and surface energy. This approach lays the foundation for fabricating heterogeneous nerve conduits with a compositional gradient along the wall thickness, which are able to promote nerve cell functions within the conduit while inhibiting cell attachment on the outer wall to prevent potential fibrous tissue formation following implantation.
我们提出了一种通过光交联甲氧基聚乙二醇单丙烯酸酯（mPEGA）与疏水性半结晶聚（ε-己内酯）二丙烯酸酯（PCLDA）在 mPEGA 的各种重量组成（ø(m))从 2 到 30%来调节表面化学和神经细胞行为的方法。由于网络中束缚的排斥性 PEG 链，表面润湿性得到改善，相应地降低了摩擦、水接触角和从细胞培养基中吸附蛋白质的能力。评估了改性表面对大鼠雪旺细胞前体细胞系（SpL201）、大鼠嗜铬细胞瘤（PC12）和 E14 小鼠神经祖细胞（NPC）的反应。这些细胞类型的细胞附着、铺展、增殖和分化与ø(m)呈非单调或抛物线依赖性，最大值在 5-7%的 ø(m)处。此外，NPC 在 mPEGA/PCLDA 网络上的ø(m)为 5%时表现出增强的神经元分化谱系，具有中等的润湿性和表面能。这种方法为制造具有沿壁厚组成梯度的异质神经导管奠定了基础，这种导管能够在导管内促进神经细胞功能，同时抑制外壁上的细胞附着，以防止植入后潜在的纤维组织形成。
