中性 pH 下锌-胰岛素的自缔合：浓度梯度——静态和动态光散射研究。
Self-association of Zn-insulin at neutral pH: investigation by concentration gradient--static and dynamic light scattering.
机构信息
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USA.
出版信息
Biophys Chem. 2010 May;148(1-3):23-7. doi: 10.1016/j.bpc.2010.02.001. Epub 2010 Feb 10.
Abstract
Equilibrium self-association of Zn-insulin at pH 7.0 was characterized over the range 0.3-5mg/mL by simultaneous measurement of static and dynamic light scattering. Analysis of static light scattering yielded a concentration-dependent weight-average molecular weight, and analysis of dynamic light scattering yielded a concentration-dependent intensity-average diffusion coefficient. The concentration dependence of both quantities may be accounted for to within experimental precision by a simple model, according to which the basic structural unit of Zn-insulin at concentrations exceeding 0.3mg/mL is a hexamer H. With increasing total protein concentration, hexameric protomers may self-associate in accordance with an isodesmic scheme in which a protomer may add to any prexisting oligomer H(n) to form H(n+1) with an invariant stepwise equilibrium association constant.
摘要
在 pH 值为 7.0 时，通过同时测量静态和动态光散射，对锌胰岛素在 0.3-5mg/mL 范围内的平衡自组装进行了表征。静态光散射分析得出了浓度依赖性的重均分子量，动态光散射分析得出了浓度依赖性的强度平均扩散系数。根据一个简单的模型，可以在实验精度范围内解释这两种数量的浓度依赖性，根据该模型，在浓度超过 0.3mg/mL 的情况下，锌胰岛素的基本结构单元是一个六聚体 H。随着总蛋白浓度的增加，六聚体原聚体可以根据等摩尔方案进行自组装，其中一个原聚体可以添加到任何预先存在的低聚物 H(n)上，以形成具有不变的逐步平衡缔合常数的 H(n+1)。
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