Institut für Photonische Technologien Jena (IPHT), Albert-Einstein-Strasse 9, 07745 Jena, Germany.
Curr Opin Biotechnol. 2010 Feb;21(1):4-11. doi: 10.1016/j.copbio.2010.02.009. Epub 2010 Mar 2.
Recent advances in sequencing technologies exhibit a tendency towards single-molecule sequencing, which eventually will lead to the commercial implementation of such platforms. For this purpose dye labelling is currently the foundation of most approaches and an overview is provided on the latest developments. For label-free sequencing the detection of conductivity changes using nanopores or nano-edges will be discussed as well as another promising method that is based on Raman spectroscopy. Here the most recent advance aims to utilize the high lateral resolution of tip-enhanced Raman scattering. For this sequencing procedure Raman spectra must be collected along the DNA or RNA strand, while the difference spectra will provide a direct sequence reading without prior labelling.
测序技术的最新进展呈现出单分子测序的趋势，最终将导致此类平台的商业化应用。为此，目前染料标记是大多数方法的基础，并提供了最新进展的概述。对于无标记测序，将讨论使用纳米孔或纳米边缘检测电导率变化，以及另一种基于拉曼光谱的有前途的方法。在这里，最新的进展旨在利用尖端增强拉曼散射的高横向分辨率。对于这种测序过程，必须沿着 DNA 或 RNA 链收集拉曼光谱，而差分光谱将提供无需预先标记的直接序列读取。
