International Center for Young Scientists National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Small. 2009 Dec;5(23):2638-49. doi: 10.1002/smll.200900976.
The growing demand for analysis of the genomes of many species and cancers, for understanding the role of genetic variation among individuals in disease, and with the ultimate goal of deciphering individual human genomes has led to the development of non-Sanger reaction-based technologies towards rapid and inexpensive DNA sequencing. Recent advancements in new DNA sequencing technologies are changing the scientific horizon by dramatically accelerating biological and biomedical research and promising an era of personalized medicine for improved human health. Two single-molecule sequencing technologies based on fluorescence detection are already in a working state. The newly launched and emerging single-molecule DNA sequencing approaches are reviewed here. The current challenges of these technologies and potential methods of overcoming these challenges are critically discussed. Further research and development of single-molecule sequencing will allow researchers to gather nearly error-free genomic data in a timely and inexpensive manner.
对许多物种和癌症基因组进行分析的需求不断增长，目的是了解个体遗传变异在疾病中的作用，最终实现对人类个体基因组的破译，这促使人们开发出基于非 Sanger 反应的快速、廉价的 DNA 测序技术。新的 DNA 测序技术的最新进展正在通过显著加快生物和生物医学研究来改变科学前景，并有望开创个性化医疗的新时代，以提高人类健康水平。两种基于荧光检测的单分子测序技术已经处于工作状态。本文综述了新推出和新兴的单分子 DNA 测序方法。本文批判性地讨论了这些技术目前面临的挑战以及克服这些挑战的潜在方法。对单分子测序的进一步研究和开发将使研究人员能够及时、廉价地获得几乎无错误的基因组数据。
