GATA School of Medicine, Department of Biochemistry and Clinical Biochemistry, Etlik, Ankara, Turkey.
Cytokine. 2010 May;50(2):192-4. doi: 10.1016/j.cyto.2010.02.008. Epub 2010 Mar 3.
Human milk is a complex biological fluid. It contains many nutrients, anti-infectious and biologically active substance. Human milk also contains many angiogenic polypeptides. We have determined four of these: Vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), insulin-like growth factor- I (IGF-I) and platelet-derived growth factor (PDGF). The aim of this study was to compare the concentrations of VEGF, b-FGF, IGF-I and PDGF in human milk collected from mothers with preterm and term neonates. Human milk samples were collected from 29 mothers of preterm (<37 weeks) and from 29 mothers of term (38>weeks) infants at days 3, 7 and 28 postpartum. Milk samples were analyzed for VEGF, b-FGF and PDGF by enzyme-linked immunosorbent assay and IGF-I was measured by radioimmunoassay method. Human milk levels of VEGF, IGF-I and b-FGF were significantly higher (p<0.001). Furthermore, within-preterm group concentrations of VEGF, IGF-I and PDGF significantly differed during postpartum days 3-7-28 (p<0.001, p<0.05, p<0.001, respectively), but did not do so for b-FGF concentrations. In term groups, concentrations of IGF-I and VEGF significantly differed (p<0.05, p<0.001, respectively), but did not do so for concentrations of b-FGF and PDGF. This is the first report of simultaneous measurements of four major angiogenic factors in human milk collected from mothers with preterm and term. Our results suggest that three of four angiogenic factors, VEGF, b-FGF and IGF-I, are higher concentration in human milk which collected from preterm mothers than those of terms.
人乳是一种复杂的生物流体。它含有许多营养物质、抗感染和生物活性物质。人乳还含有许多血管生成多肽。我们已经确定了其中的四种：血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（b-FGF）、胰岛素样生长因子-I（IGF-I）和血小板衍生生长因子（PDGF）。本研究的目的是比较早产儿（<37 周）和足月儿（38>周）母亲产后第 3、7 和 28 天收集的人乳中 VEGF、b-FGF、IGF-I 和 PDGF 的浓度。从 29 名早产儿（<37 周）和 29 名足月儿（38>周）母亲产后第 3、7 和 28 天收集人乳样本。通过酶联免疫吸附试验分析 VEGF、b-FGF 和 PDGF，放射免疫法测量 IGF-I。人乳 VEGF、IGF-I 和 b-FGF 水平明显较高（p<0.001）。此外，在早产儿组中，产后第 3-7-28 天 VEGF、IGF-I 和 PDGF 的浓度差异有统计学意义（p<0.001、p<0.05、p<0.001），但 b-FGF 浓度无差异。在足月儿组中，IGF-I 和 VEGF 浓度差异有统计学意义（p<0.05、p<0.001），但 b-FGF 和 PDGF 浓度无差异。这是首次报告同时测量来自早产儿和足月儿母亲的人乳中四种主要血管生成因子。我们的结果表明，四种血管生成因子中的三种，VEGF、b-FGF 和 IGF-I，在早产儿母亲的人乳中的浓度高于足月母亲的人乳。
