Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, PR China.
J Antibiot (Tokyo). 2010 Apr;63(4):187-9. doi: 10.1038/ja.2010.16. Epub 2010 Mar 5.
Quinolones are used extensively to treat Shigella flexneri infection, and plasmid-mediated quinolone resistance (PMQR) determinants were recently reported. A total of 26 S. flexneri isolates collected from Anhui province, China, in 2005 were screened for PMQR determinants, bla gene, gyrA and parC genes, by PCR and sequencing. PMQR determinants were present in 53.8% (14 of 26) of isolates and qnrA(1), qnrS(1), qnrS(2), aac(6')-Ib-cr and qepA were present in 30.8, 11.5, 3.8, 19.2 and 11.5% of those isolates, respectively. All PMQR determinants' positive isolates exhibiting 8 different genetic clones had mutations in gyrA and parC genes, and 11 carried bla genes, including 7 bla(CTX-M) with resistance to cefotaxime. These isolates were resistant to nalidixic acid and 57.1% (8 of 14) of them were resistant to ciprofloxacin and levofloxacin. Our data show that there was a high prevalence of PMQR determinants in S. flexneri isolates from China.
喹诺酮类药物被广泛用于治疗福氏志贺菌感染，最近报道了质粒介导的喹诺酮类耐药（PMQR）决定因素。从 2005 年中国安徽省采集的 26 株福氏志贺菌分离株通过 PCR 和测序筛选 PMQR 决定因素、bla 基因、gyrA 和 parC 基因。在 53.8%（26 株中的 14 株）的分离株中存在 PMQR 决定因素，qnrA(1)、qnrS(1)、qnrS(2)、aac(6')-Ib-cr 和 qepA 的检出率分别为 30.8%、11.5%、3.8%、19.2%和 11.5%。所有 PMQR 决定因素阳性的分离株均表现出 8 种不同的遗传克隆，gyrA 和 parC 基因发生突变，11 株携带 bla 基因，包括 7 株 bla(CTX-M)对头孢噻肟具有耐药性。这些分离株对萘啶酸耐药，57.1%（14 株中的 8 株）对环丙沙星和左氧氟沙星耐药。我们的数据表明，中国福氏志贺菌分离株中 PMQR 决定因素的流行率很高。
